Development of Novel Wheat-Aegilops longissima 3S1 Translocations Conferring Powdery Mildew Resistance and Specific Molecular Markers for Chromosome 3S1
Development of Novel Wheat-Aegilops longissima 3S1 Translocations Conferring Powdery Mildew Resistance and Specific Molecular Markers for Chromosome 3S1
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DOI：
10.1094/pdis-12-20-2691-re
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发表时间：
2021-10-01
期刊：
影响因子：
4.5
通讯作者：
Liu, Wenxuan
中科院分区：
文献类型：
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作者：
Li, Huanhuan;Tian, Xiubin;Liu, Wenxuan
Y小麦白粉病，由Blumeria graminis f.小麦赤霉病是小麦的一种毁灭性病害。培育抗病品种是最具成本效益的疾病管理策略。中国春（CS）-长山羊草（Aegilops longissima）3S1#2（3B）二体代换系TA 3575的染色体3S（1）#2具有抗白粉病的特性。本研究通过对B的鉴定，进一步将抗白粉病基因定位在染色体3S（1）#2（3S（1）#2S）短臂上。禾本科植物新开发的CS-Ae. longissima 3S（1）#2易位系。与此同时，TA 7545，以前指定的CS-Ae。longissima 3S（1）#3二体附加系重新鉴定为等染色体3S（1）#3S附加系，并评价其赋予对白粉病的抗性，从而将抗性基因定位于染色体3S（1）#3的短臂（3S（1）#3S）。基于TA 3575的转录组序列，开发了10个新的染色体3S（1）S特异性标记，其中5个可用于区分来自Ae的3S（1）#2S和3S（1）#3S。另外5份既能识别3S（1）#2S又能识别3S（1）#3S。CL 897是对3S（1）#2S和3S（1）#3S都具有特异性的5个标记之一，可用于检测位于3S（1）#1S染色体上的Pm 13。longissima登录号TL 01在不同小麦遗传背景中的遗传多样性。3S（1）#2S和3S（1）#3S染色体上的抗白粉病基因，CS-Ae. longissima 3S（1）#2易位系，本研究开发的3S（1）S特异标记将有助于B的转移。禾本科植物将小麦抗白粉病基因导入普通小麦，为小麦抗白粉病育种提供新的种质资源。
Y Powdery mildew of wheat, caused by Blumeria graminis f. sp. tritici, is a destructive disease of common wheat. Cultivation of resistant varieties is the most cost-effective disease management strategy. Previous studies reported that chromosome 3S(1)#2 present in Chinese Spring (CS)-Aegilops longissima 3Sl #2(3B) disomic substitution line TA3575 conferred resistance to powdery mildew. In this study, we further located the powdery mildew resistance gene(s) to the short arm of chromosome 3S(1)#2 (3S(1)#2S) by evaluating for B. graminis f. sp. tritici resistance of newly developed CS-Ae. longissima 3S(1)#2 translocation lines. Meanwhile, TA7545, a previously designated CS-Ae. longissima 3S(1)#3 disomic addition line, was reidentified as an isochromosome 3S(1)#3S addition line and evaluated to confer resistance to powdery mildew, thus locating the resistance gene(s) to the short arm of chromosome 3S(1)#3 (3S(1)#3S). Based on transcriptome sequences of TA3575, 10 novel chromosome 3S(1) S-specific markers were developed, of which 5 could be used to distinguish between 3S(1)#2S and 3S(1)#3S derived from Ae. longissima accessions TL20 and TA1910 (TAM4) and the remaining 5 could identify both 3S(1)#2S and 3S(1)#3S. Also, CL897, one of five markers specific to both 3S(1)#2S and 3S(1)#3S, could be used to detect Pm13 located at chromosome 3S(1)#1S from Ae. longissima accession TL01 in diverse wheat genetic backgrounds. The powdery mildew resistance genes on chromosomes 3S(1)#2S and 3S(1)#3S, the CS-Ae. longissima 3S(1)#2 translocation lines, and the 3S(1) S-specific markers developed in this study will facilitate the transfer of B. graminis f. sp. tritici resistance genes into common wheat and provide new germplasm resources for powdery mildew resistance breeding.