RESPONSES OF DENERVATED ADULT NEWT LIMB STUMPS TO RE-INNERVATION AND RE-INJURY
RESPONSES OF DENERVATED ADULT NEWT LIMB STUMPS TO RE-INNERVATION AND RE-INJURY
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DOI：
10.1002/jez.1402150208
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发表时间：
1981-01-01
影响因子：
--
通讯作者：
TASSAVA, RA
中科院分区：
文献类型：
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作者：
SALLEY, JD;TASSAVA, RA
失去神经支配的成年蝾螈四肢不能再生。目前还不清楚为什么去神经蝾螈肢残端不能启动再生后，神经再支配。为了确定再生的限制因素，对长期失神经支配的蝾螈肢体残端进行了组织学检查，并测试了各种再损伤的影响。失神经支配5周的断肢可见去分化细胞、分化软骨和密集的层状细胞。覆盖肢体尖端的表皮类似皮肤表皮而非伤口表皮。成功恢复5周失神经支配肢体残端再生能力的再损伤类型包括再截肢（100%），单个剃刀切口（60%）和从远端肢体尖端切除愈合组织（70%）。强调了损伤和创伤表皮在启动再生中的重要性。
Denervated adult newt limbs do not regenerate. It is not understood why denervated newt limb stumps fail to initiate regeneration upon reinnervation. To define the regeneration limiting factors, the histology of long-term denervated newt limb stumps were examined and the effects of various kinds of reinjury were tested. In 5 wk denervated limb stumps there were present dedifferentiated cells, differentiated cartilage and densely packed layered cells. The epidermis covering the limb tip resembled skin epidermis rather than wound epidermis. The kinds of reinjury that were successful in restoring the regeneration capabilities to 5 wk denervated limb stumps included reamputation (100%), a single razor incision (60%) and removal of the healed tissues from the distal limb tip (70%). The importance of injury and wound epidermis in initiating regeneration is emphasized.