RNA structures and dynamics with Å resolution revealed by x-ray free-electron lasers.
RNA structures and dynamics with Å resolution revealed by x-ray free-electron lasers.
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DOI：
10.1126/sciadv.adj3509
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发表时间：
2023-09-29
期刊：
影响因子：
13.6
通讯作者：
Pollack, Lois
中科院分区：
文献类型：
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作者：
Zielinski, Kara A.;Sui, Shuo;Pabit, Suzette A.;Rivera, Daniel A.;Wang, Tong;Hu, Qingyue;Kashipathy, Maithri M.;Lisova, Stella;Schaffer, Chris B.;Mariani, Valerio;Hunter, Mark S.;Kupitz, Christopher;Moss III, Frank R.;Poitevin, Frederic P.;Grant, Thomas D.;Pollack, Lois
RNA大分子，如蛋白质，折叠成与其广泛认可的生物功能密切相关的形状;然而，由于其高电荷和动态性质，RNA结构的确定更具挑战性。我们介绍了一种方法，利用高亮度的X射线自由电子激光源，揭示了结构化和非结构化RNA的埃尺度特征的形成和识别。通过广角溶液散射实验识别了以前未被识别的RNA二级和三级结构的结构特征。与毫秒的时间分辨率，我们观察到一个RNA折叠从一个动态变化的单链通过一个碱基配对的中间体，承担三螺旋构象。当骨架协调折叠时，最终的结构被基础堆叠锁定。这种方法可以帮助快速表征和识别核酸中的结构元件在平衡和时间分辨实验。动态变化的RNA结构可视化的时间分辨，高分辨率的溶液散射实验。
RNA macromolecules, like proteins, fold to assume shapes that are intimately connected to their broadly recognized biological functions; however, because of their high charge and dynamic nature, RNA structures are far more challenging to determine. We introduce an approach that exploits the high brilliance of x-ray free-electron laser sources to reveal the formation and ready identification of angstrom-scale features in structured and unstructured RNAs. Previously unrecognized structural signatures of RNA secondary and tertiary structures are identified through wide-angle solution scattering experiments. With millisecond time resolution, we observe an RNA fold from a dynamically varying single strand through a base-paired intermediate to assume a triple-helix conformation. While the backbone orchestrates the folding, the final structure is locked in by base stacking. This method may help to rapidly characterize and identify structural elements in nucleic acids in both equilibrium and time-resolved experiments. Dynamically changing RNA structures are visualized in time-resolved, high-resolution solution scattering experiments.
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影响因子：
7.4
作者：
Calvey, George D.;Katz, Andrea M.;Pollack, Lois
通讯作者：
Pollack, Lois
影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
影响因子：
8.9
作者：
Chen, Yen-Lin;He, Weiwei;Pollack, Lois
通讯作者：
Pollack, Lois
蜡和MD揭示的核酸双链体的结构可塑性。
影响因子：
13.6
作者：
He W;Chen YL;Pollack L;Kirmizialtin S
通讯作者：
Kirmizialtin S
影响因子：
8.4
作者：
通讯作者：
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