Photoelectrochemical imaging system with high spatiotemporal resolution for visualizing dynamic cellular responses.
Photoelectrochemical imaging system with high spatiotemporal resolution for visualizing dynamic cellular responses.
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DOI：
10.1016/j.bios.2021.113121
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发表时间：
2021-03
影响因子：
12.6
通讯作者：
Bo Zhou;Anirban Das;Muchun Zhong;Qian Guo;De-Wen Zhang;K. Hing;A. Sobrido;M. Titirici;S. Krause
中科院分区：
文献类型：
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作者：
Bo Zhou;Anirban Das;Muchun Zhong;Qian Guo;De-Wen Zhang;K. Hing;A. Sobrido;M. Titirici;S. Krause
光电化学成像在细胞过程的无标记研究中具有巨大的潜力。在这里，我们报道了一种新的快速光电化学成像系统(PEIS)，用于活细胞的直流光电流成像，它结合了高速、出色的横向分辨率和高光电流稳定性，这些都是研究动态细胞过程的关键。采用模拟微镜对传感器基板进行光栅化处理，实现高速成像。采用电沉积法制备的α-Fe_2O_3(赤铁矿)薄膜作为坚固的衬底，具有较高的光电流和良好的空间分辨率。通过监测细胞对Triton X-100通透性的反应，证明了该系统的能力。与前面描述的较慢的电化学单电池成像技术相比，同时执行多个电池的动态功能成像的能力提供了对数据的更高的置信度。当监测pH值变化时，PEIS可以达到每秒8帧的帧速率。
Photoelectrochemical imaging has great potential in the label-free investigation of cellular processes. Herein, we report a new fast photoelectrochemical imaging system (PEIS) for DC photocurrent imaging of live cells, which combines high speed with excellent lateral resolution and high photocurrent stability, which are all crucial for studying dynamic cellular processes. An analog micromirror was adopted to raster the sensor substrate, enabling high-speed imaging. α-Fe2O3(hematite) thin films synthesized via electrodeposition were used as a robust substrate with high photocurrent and good spatial resolution. The capabilities of this system were demonstrated by monitoring cell responses to permeabilization with Triton X-100. The ability to carry out dynamic functional imaging of multiple cells simultaneously provides improved confidence in the data than could be achieved with the slower electrochemical single-cell imaging techniques described previously. When monitoring pH changes, the PEIS can achieve frame rates of 8 frames per second.