Characterization of nanoprobe uptake in single cells: spatial and temporal tracking via SERS labeling and modulation of surface charge.
Characterization of nanoprobe uptake in single cells: spatial and temporal tracking via SERS labeling and modulation of surface charge.
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DOI：
10.1016/j.nano.2010.07.009
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发表时间：
2011-02
期刊：
影响因子：
--
通讯作者：
Vo-Dinh T
中科院分区：
文献类型：
--
作者：
Gregas MK;Yan F;Scaffidi J;Wang HN;Vo-Dinh T
在生物医学研究和临床应用中使用纳米探针的一个关键方面涉及它们在细胞中的摄取和分布的基本空间和时间表征。拉曼光谱和二维拉曼成像被用于使用表面增强拉曼散射检测来识别和定位单细胞中的纳米探针。为了研究细胞吸收的效率，将用三种不同的正电荷、负电荷和中性电荷拉曼标记物功能化的银纳米颗粒与细胞培养物共孵育，并通过正常细胞过程内化。观察到纳米颗粒上的表面电荷调节摄取效率，证明了作为跟踪标记和作为细胞摄取调节剂的表面修饰的双重功能。这些结果表明，功能化的纳米颗粒结构具有在单个活细胞中感测和递送的潜力，并且使用表面增强的拉曼散射进行跟踪和检测是传统荧光方法的实用且有利的替代方案。
A critical aspect for use of nanoprobes in biomedical research and clinical applications involves fundamental spatial and temporal characterization of their uptake and distribution in cells. Raman spectroscopy and two-dimensional Raman imaging were used to identify and locate nanoprobes in single cells using surface-enhanced Raman scattering detection. To study the efficiency of cellular uptake, silver nanoparticles functionalized with three different positive-, negative-, and neutrally charged Raman labels were co-incubated with cell cultures and internalized via normal cellular processes. The surface charge on the nanoparticles was observed to modulate uptake efficiency, demonstrating a dual function of the surface modifications as tracking labels and as modulators of cell uptake. These results indicate that the functionalized nanoparticle construct has potential for sensing and delivery in single living cells and that use of surface-enhanced Raman scattering for tracking and detection is a practical and advantageous alternative to traditional fluorescence methods.