The microbiome composition of Aedes aegypti is not critical for Wolbachia-mediated inhibition of dengue virus
The microbiome composition of Aedes aegypti is not critical for Wolbachia-mediated inhibition of dengue virus
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DOI：
10.1371/journal.pntd.0005426
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发表时间：
2017-03-01
影响因子：
3.8
通讯作者：
McGraw, Elizabeth A.
中科院分区：
文献类型：
--
作者：
Audsley, Michelle D.;Ye, Yixin H.;McGraw, Elizabeth A.
背景登革病毒（DENV）主要由埃及伊蚊（Aedesaegypti）传播，估计每年造成3.9亿人感染。DENV控制的一种新方法涉及Ae的稳定转染。埃及伊蚊与常见昆虫内共生体沃尔巴克氏体，介导抗病毒作用。然而，Wolbachia降低Ae.埃及人感染登革病毒的原因尚不完全清楚。在这项研究中，我们评估了潜在的居民微生物群，这可以在昆虫生理学和免疫反应中发挥重要作用，影响沃尔巴克氏体介导的DENV阻断。将稳定感染沃尔巴克氏体菌株wMel的埃及伊蚊与Ae.不含Wolbachia的埃及伊蚊，使用16 s rDNA分析。我们的研究结果表明，尽管沃尔巴克氏体影响了几个属的相对丰度，但沃尔巴克氏体感染和未感染蚊子的微生物组均以嗜热金氏体和未分类的肠杆菌科为主。为了评估驻留微生物群影响沃尔巴克氏体介导的抗病毒作用的潜力，我们在通过血餐感染DENV之前使用抗生素治疗。尽管微生物组组成的显着转变，在响应抗生素，我们检测到没有效果的抗生素治疗DENV感染率，或感染mosquitoes.Conclusions/SignificanceOur的研究结果表明，稳定的感染与沃尔巴克氏体菌株wMel实验室饲养的Ae的微生物组产生的影响不大。埃及人。此外，我们的研究结果表明，微生物组可以显着改变，而不影响这些蚊子中的基本DENV阻断表型。自从Ae.埃及伊蚊可能在田间遇到不同的微生物群，这在使用沃尔巴克氏体作为DENV控制方法的背景下是特别重要的结果。
BackgroundDengue virus (DENV) is primarily vectored by the mosquito Aedes aegypti, and is estimated to cause 390 million human infections annually. A novel method for DENV control involves stable transinfection of Ae. aegypti with the common insect endosymbiont Wolbachia, which mediates an antiviral effect. However, the mechanism by which Wolbachia reduces the susceptibility of Ae. aegypti to DENV is not fully understood. In this study we assessed the potential of resident microbiota, which can play important roles in insect physiology and immune responses, to affect Wolbachia-mediated DENV blocking.Methodology/FindingsThe microbiome of Ae. aegypti stably infected with Wolbachia strain wMel was compared to that of Ae. aegypti without Wolbachia, using 16s rDNA profiling. Our results indicate that although Wolbachia affected the relative abundance of several genera, the microbiome of both the Wolbachia-infected and uninfected mosquitoes was dominated by Elizabethkingia and unclassified Enterobacteriaceae. To assess the potential of the resident microbiota to affect the Wolbachia-mediated antiviral effect, we used antibiotic treatment before infection with DENV by blood-meal. In spite of a significant shift in the microbiome composition in response to the antibiotics, we detected no effect of antibiotic treatment on DENV infection rates, or on the DENV load of infected mosquitoes.Conclusions/SignificanceOur findings indicate that stable infection with Wolbachia strain wMel produces few effects on the microbiome of laboratory-reared Ae. aegypti. Moreover, our findings suggest that the microbiome can be significantly altered without affecting the fundamental DENV blocking phenotype in these mosquitoes. Since Ae. aegypti are likely to encounter diverse microbiota in the field, this is a particularly important result in the context of using Wolbachia as a method for DENV control.