A CDNA FRAGMENT OF HEPATITIS-C VIRUS ISOLATED FROM AN IMPLICATED DONOR OF POST-TRANSFUSION NON-A, NON-B HEPATITIS IN JAPAN
A CDNA FRAGMENT OF HEPATITIS-C VIRUS ISOLATED FROM AN IMPLICATED DONOR OF POST-TRANSFUSION NON-A, NON-B HEPATITIS IN JAPAN
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DOI：
10.1093/nar/17.24.10367
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发表时间：
1989-12-25
影响因子：
14.9
通讯作者：
MIYAMURA, T
中科院分区：
文献类型：
--
作者：
KUBO, Y;TAKEUCHI, K;MIYAMURA, T
最近，在美国从慢性感染的黑猩猩中分离出丙型肝炎病毒（HCV）RNA基因组的cDNA。为了分离来自人材料的HCV cDNA，从输血后非甲非乙型肝炎的日本献血者的血浆中提取RNA，合成HCV cDNA，并使用HCV特异性寡核苷酸引物通过PCR方法扩增。该cDNA片段长583个核苷酸，与HCV原型cDNA的核苷酸同源性为79.8%，氨基酸同源性为92.2%。这些结果进一步证明HCV与输血后非甲非乙型肝炎的发生密切相关。
Recently, a cDNA from the hepatitis C virus (HCV) RNA genome has been isolated in the USA from a chronically infected chimpanzee. In order to isolate HCV cDNA derived from human material, RNA was extracted from plasma of a Japanese blood donor implicated in post-transfusion non-A, non-B hepatitis and HCV cDNA was synthesized and amplified by the PCR method using HCV-specific oligonucleotide primers. The cDNA fragment, 583 nucleotides long, showed 79.8% homology at the nucleotide level and 92.2% homology at the amino acid level compared with the prototype HCV cDNA. These results provides further evidence to show that HCV is closely associated with the development of post transfusion non-A, non-B hepatitis.