Time course of bioluminescent signal in orthotopic and heterotopic brain tumors in nude mice
Time course of bioluminescent signal in orthotopic and heterotopic brain tumors in nude mice
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DOI：
10.2144/03346st01
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发表时间：
2003-06-01
期刊：
影响因子：
2.7
通讯作者：
Laug, WE
中科院分区：
文献类型：
--
作者：
Burgos, JS;Rosol, M;Laug, WE
活体生物发光成像正变得越来越流行。生物发光信号的量化需要了解这项技术的可变性和重复性。本研究的目的是分析萤火虫荧光素酶表达肿瘤在两个位置、注射荧光素后和肿瘤细胞植入后不同时间的发光信号的时间历程。在纵向研究期间，为了可靠地量化和比较信号，需要了解生物发光信号的动力学知识。利用一种结构性表达荧光素酶的人脑肿瘤细胞系，对小鼠原位和异位脑肿瘤的生物发光动力学进行了研究。将肿瘤细胞植入动物的大脑和侧翼，获得揭示肿瘤位置的全身图像，通过光子发射定量监测肿瘤负荷随时间的变化。体内测量的生物发光强度在注射荧光素后随着时间和剂量的变化而变化，这就要求定量结果的比较必须考虑到注射后的时间。异位肿瘤和原位肿瘤表现出显着不同的时间进程；然而，以细胞植入后第4天和第14天的动力学研究为特征的植入后时间在原位肿瘤中没有显着差异。未来的定量纵向研究必须考虑到不同模型的动力学差异。
In vivo bioluminescence imaging is becoming increasingly popular. Quantification of bioluminescent signals requires knowledge of the variability and reproducibility of this technique. The objective of this study was to analyze the time course of luminescent signal emitted from firefly luciferase-expressing tumors in two locations, following luciferin injection and at different times after tumor cell implantation. Knowledge of the kinetics of the bioluminescent signals is required for the reliable quantification and comparison of signal during longitudinal studies. The kinetics of bioluminescence was evaluated in orthotopic and heterotopic brain tumors in mice using a human brain tumor cell line constitutively expressing luciferase. Tumor cells were implanted in the brains and flanks of the animals, and whole body images revealing tumor location were obtained Tumor burden was monitored over time by the quantitation of photon emission. The magnitude of bioluminescence measured in vivo varied with time after the injection of luciferin, as well as with dose, which necessitated that the comparison of the quantitative results take into consideration the time after injection. Heterotopic and orthotopic tumors exhibited significantly different time courses; however time after implantation as characterized by kinetic studies performed on days 4 and 14 after cell implantation revealed no significant differences in orthotopic tumors. Future quantitative longitudinal studies must take into account the differences in the kinetics of different models.