Comparison of the Effectiveness of Three Irrigation Techniques in Reducing Intracanal Enterococcus faecalis Populations: An In Vitro Study
Comparison of the Effectiveness of Three Irrigation Techniques in Reducing Intracanal Enterococcus faecalis Populations: An In Vitro Study
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DOI：
10.1016/j.joen.2009.07.001
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发表时间：
2009-10-01
影响因子：
4.2
通讯作者：
Siqueira, Jose F., Jr.
中科院分区：
文献类型：
--
作者：
Brito, Patricia R. R.;Souza, Leticia C.;Siqueira, Jose F., Jr.
介绍：最近引入了几种冲洗技术，主要目的是改善根管消毒。本体外研究旨在比较化学机械预备与3种不同冲洗技术促进根管内细菌减少的效果。研究方法：用粪肠球菌ATCC 29212污染拔出牙齿的根管7天，然后随机分配到3个实验组，每组20颗牙齿：第1组，使用NaviTip针头插入工作长度短3 mm的常规冲洗;第2组，与第1组相同，但补充了EndoActivator系统的最终冲洗液激活;第3组，使用EndoVac系统冲洗。所有实验组均采用NaOCl和乙二胺四乙酸（EDTA）冲洗液。各组的总体制备时间保持不变，但总体积范围为20 mL（组1和2）至43 mL（组3）。对照组用生理盐水冲洗（总体积，43 mL）。在化学机械程序之前和之后采集的样品进行培养，并对集落形成单位（CFU）进行计数。结果：所有组的细菌种群均显著减少。以NaOCl和EDTA为冲洗液的3个实验组在降低CFU计数方面均显著优于以生理盐水为对照组。测试的3种技术之间没有显著差异。结论：在当前体外模型中评估的任何冲洗技术都没有明显的抗菌优势。（J Endod 2009;35：1422-1427）
Introduction: Several irrigation techniques have been recently introduced with the main objective of improving root canal disinfection. This in vitro study aimed at comparing the intracanal bacterial reduction promoted by chemomechanical preparation with 3 different irrigation techniques. Methods: Root canals from extracted teeth were contaminated with Enterococcus faecalis ATCC 29212 for 7 days and then randomly distributed into 3 experimental groups of 20 teeth each: group 1, conventional irrigation with NaviTip needles inserted up to 3 mm short of the working length; group 2, same as group 1, but supplemented with final irrigant activation by the EndoActivator system; and group 3, irrigation with the EndoVac system. NaOCl and ethylenediaminetetraacetic acid (EDTA) were the irrigants used in all experimental groups. The overall preparation time was kept constant for the groups, but the total volume ranged from 20 mL (groups 1 and 2) to 43 mL (group 3). The control group was irrigated with saline solution (total volume, 43 mL). Samples taken before and after chemomechanical procedures were cultured, and the colony-forming units (CFUs) were counted. Results: Reduction in the bacterial populations was highly significant for all groups. The 3 experimental groups with NaOCl and EDTA as irrigants were significantly more effective than the control group with saline in reducing CFU counts. There were no significant differences between the 3 techniques tested. Conclusions: There was no evident antibacterial superiority of any of the irrigation techniques evaluated in the present in vitro model. (J Endod 2009;35:1422-1427)