Role of the nuclease activities encoded by herpes simplex virus 1 UL12 in viral replication and neurovirulence.
Role of the nuclease activities encoded by herpes simplex virus 1 UL12 in viral replication and neurovirulence.
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单纯疱疹病毒 1 UL12 编码的核酸酶活性在病毒复制和神经毒力中的作用。
DOI：
10.1128/jvi.03621-13
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发表时间：
2014
影响因子：
5.4
通讯作者：
Kawaguchi Y.
中科院分区：
文献类型：
--
作者：
Fujii H;Mugitani M;Koyanagi N;Liu Z;Tsuda S;Arii J;Kato A;Kawaguchi Y.
单纯疱疹病毒 1 UL12 基因中的酶死突变消除了核酸内切酶和核酸外切酶活性，仅略微降低了细胞培养物中的病毒复制。然而，UL12无效突变显着降低了病毒复制，表明与其核酸酶活性无关的UL12功能在病毒复制中发挥了主要作用。相比之下，酶死亡突变显着降低了小鼠体内的病毒神经毒力，表明 UL12 核酸酶活性对于体内病毒发病机制至关重要。
Enzyme-dead mutations in the herpes simplex virus 1 UL12 gene that abolished its endo- and exonuclease activities only slightly reduced viral replication in cell cultures. However, the UL12 null mutation significantly reduced viral replication, suggesting that a UL12 function(s) unrelated to its nuclease activities played a major role in viral replication. In contrast, the enzyme-dead mutations significantly reduced viral neurovirulence in mice, suggesting that UL12 nuclease activities were critical for viral pathogenesisin vivo.