Expression of Epithelial-Mesenchymal Transition Proteins in Pancreatic Anaplastic (Undifferentiated) Carcinoma.
Expression of Epithelial-Mesenchymal Transition Proteins in Pancreatic Anaplastic (Undifferentiated) Carcinoma.
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DOI：
10.1097/mpa.0000000000001199
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发表时间：
2019-01
期刊：
影响因子：
2.9
通讯作者：
Sugai T
中科院分区：
文献类型：
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作者：
Ishida K;Yamashita R;Osakabe M;Uesugi N;Yamada N;Nitta H;Fujishima F;Motoi F;Suzuki H;Shimamura H;Noda Y;Sawai T;Unno M;Sasano H;Sasaki A;Sugai T
文本中提供了补充数字内容。本研究的目的是确定胰腺间变性癌（APC）与上皮间质转化（EMT）之间的关联。检查切除的 APC（n = 24）以评估 APC 的组成部分，包括癌性、移行性和肉瘤性区域。基于 E-钙粘蛋白和 3 个 EMT 相关蛋白的免疫反应性进行分析：Slug（锌指蛋白 SNAI2）、Twist（Twist 相关蛋白 1）和 Zeb1（锌指 E 盒结合同源盒 1）。根据靶细胞的染色强度和染色面积确定表达评分。最后，我们根据肉瘤成分的 E-钙粘蛋白和 EMT 相关蛋白的表达模式进行层次聚类。 E-cadherin表达评分按肉瘤>移行>癌性成分依次降低（P < 0.01）。尽管癌性成分和移行性成分之间 Slug、Twist 和 Zeb1 的免疫组化评分存在显着性差异（P < 0.01），但移行性成分和肉瘤性成分之间 Zeb1 的免疫组化评分存在显着性差异（P < 0.05）。此外，根据E-钙粘蛋白和EMT相关蛋白的表达模式（层次聚类分析）将APC分为2个亚组。因此，这些亚组通过 Twist 表达来区分。上皮-间质转化在 APC 的发病机制中起着重要作用。
Supplemental digital content is available in the text. The aim of this study was to identify an association of pancreatic anaplastic carcinoma (APC) with the epithelial-mesenchymal transition (EMT). Resected APCs (n = 24) were examined to assess components of APCs, including carcinomatous, transitional, and sarcomatous regions. Analysis was performed based on the immunoreactivity of E-cadherin and 3 EMT-related proteins: Slug (zinc finger protein SNAI2), Twist (Twist-related protein 1), and Zeb1 (zinc finger E-box–binding homeobox 1). Expression score was determined based on staining intensity and stained area of the target cells. Finally, we performed a hierarchical clustering based on the expression pattern of E-cadherin and EMT-related proteins of the sarcomatous component. The expression score of E-cadherin decreased in the order of sarcomatous > transitional > carcinomatous components (P < 0.01). Although there were significant differences in the immunohistochemical scores of Slug, Twist, and Zeb1 between carcinomatous and transitional components (P < 0.01), the significant difference in immunohistochemical score of Zeb1 between transitional and sarcomatous components was found (P < 0.05). Furthermore, APCs were divided into 2 subgroups based on the expression patterns of E-cadherin and EMT-related proteins (hierarchical clustering analysis). Consequently, these subgroups were distinguished by Twist expression. Epithelial-mesenchymal transition plays an essential role in the pathogenesis of APC.