A comparison of lipase‐catalyzed ester hydrolysis in reverse micelles, organic solvents, and biphasic systems
A comparison of lipase‐catalyzed ester hydrolysis in reverse micelles, organic solvents, and biphasic systems
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DOI：
10.1002/bit.260470108
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发表时间：
1995-07
影响因子：
3.8
通讯作者：
Fangxiao Yang;A. Russell
中科院分区：
文献类型：
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作者：
Fangxiao Yang;A. Russell
以醋酸乙酯、醋酸烯丙酯和醋酸戊酯为模型反应，研究了产自白色念珠菌和小麦胚芽的脂肪酶在三种反应介质中的性能。研究了各体系（有机溶剂体系、双相体系和反胶束体系）中基质性质和含水量的影响。不出所料，含水量对每个系统的影响是不同的，酶活性的最佳含水量并不总是与生产力的最佳含水量相同。用理论模型模拟和预测了酶在反胶束中的性能，所提出的双相体系分配模型与实验结果吻合较好。虽然在胶束体系中观察到最高的活性，但微乳的生产力受到低酶浓度的限制。然而，双相系统支持相对较好的活动和生产力。在干燥的有机溶剂中加入水，再加上冻干酶粉在溶剂中的分散，导致了显著的酶聚集，这就限制了“无水”酶悬浮方法的适用性。©1995 John Wiley & Sons, Inc
The performance of lipases from Candida rugosa and wheat germ have been investigated in three reaction media using three acetate hydrolyses as model reactions (ethyl acetate, allyl acetate, and prenyl acetate). The effect of substrate properties and water content were studied for each system (organic solvent, biphasic system, and reverse micelles). Not unexpectedly, the effect of water content is distinct for each system, and the optimal water content for enzyme activity is not always the same as that for productivity. A theoretical model has been used to simulate and predict enzyme performance in reverse micelles, and a proposed partitioning model for biphasic systems agrees well with experimental results. While the highest activities observed were in the micellar system, productivity in microemulsions is limited by low enzyme concentrations. Biphasic systems, however, support relatively good activity and productivity. The addition of water to dry organic solvents, combined with the dispersion of lyophilized enzyme powders in the solvent, resulted in significant enzyme aggregation, which not surprisingly limits the applicability of the “anhydrous” enzyme suspension approach. © 1995 John Wiley & Sons, Inc.