Hymenoptera-venom-induced upregulation of the basophil activation marker ecto-nucleotide pyrophosphatase/phosphodiesterase 3 in sensitized individuals
Hymenoptera-venom-induced upregulation of the basophil activation marker ecto-nucleotide pyrophosphatase/phosphodiesterase 3 in sensitized individuals
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DOI：
10.1159/000049531
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发表时间：
2001-12-01
影响因子：
2.8
通讯作者：
Bühring, HJ
中科院分区：
文献类型：
--
作者：
Platz, IJ;Binder, M;Bühring, HJ
背景。蜜蜂和黄蜂的毒液是能引起严重临床反应的强效过敏原。为了检测对这些过敏原的直接型超敏反应，开发了一种快速的体外试验，该试验依赖于活化的嗜碱性细胞上的外核苷酸焦磷酸酶/磷酸二酯酶3 （E-NPP3）的上调。方法：采用97A6单克隆抗体检测13例健康献血者和22例蜂毒过敏患者血液中E-NPP3 （CD203c）的表达。用抗ige抗体或过敏原激活后，流式细胞术检测嗜碱性粒细胞。将毒液诱导的E-NPP3对嗜碱性粒细胞的上调与诊断参数（包括皮肤试验和特异性IgE评估）进行比较。在选定的样本中，将活化的嗜碱性细胞上E-NPP3表达的增加与组胺释放和CD63上调进行比较。结果：在20/22的黄蜂或蜂毒致敏患者中，E-NPP3在变态反应原或抗ige激活下表达上调。在1杯/ml过敏原或抗ige抗体刺激15分钟后，E-NPP3表达量最大（高于基线的10倍）。致敏个体在响应IgE受体交联时未能上调E-NPP3，也未能诱导组胺释放和CD63上调。结论：流式细胞术检测膜翅虫毒液诱导的E-NPP3上调是一种新的体外检测致敏个体的方法。版权所有(C) 2002 S. Karger AG，巴塞尔。
Background. Bee and wasp venoms are potent allergens capable of inducing severe clinical reactions. To detect immediate-type hypersensitivity to these allergens, a rapid in vitro test was developed that relies on the upregulation of ecto-nucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP3) on activated basophils. Methods: Blood basophils of 13 healthy donors and 22 patients allergic to bee or wasp venom were analyzed for E-NPP3 (CD203c) expression using monoclonal antibody 97A6. Basophils were analyzed by flow cytometry after activation with anti-IgE antibody or allergen. Venom-induced E-NPP3 upregulation on basophils was compared with diagnostic parameters, including skin tests and assessment of specific IgE. In selected samples, the increase in E-NPP3 expression on activated basophils was compared with histamine release and CD63 upregulation. Results: In 20/22 patients sensitized to wasp or bee venom, E-NPP3 expression on basophils was upregulated in response to activation by allergen or anti-IgE. The maximum increase in E-NPP3 expression (above ten times of baseline) was achieved after 15 min of stimulation with 1 mug/ml of allergen or anti-IgE antibody. Sensitized individuals who failed to upregulate E-NPP3 in response to IgE receptor cross-linking also failed to induce histamine release and CD63 upregulation. Conclusions: Flow cytometric determination of hymenoptera-venom-induced upregulation of E-NPP3 is a novel in vitro test to identify sensitized individuals. Copyright (C) 2002 S. Karger AG, Basel.