VARIATION IN A MAJOR SURFACE PROTEIN OF LYME-DISEASE SPIROCHETES
VARIATION IN A MAJOR SURFACE PROTEIN OF LYME-DISEASE SPIROCHETES
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DOI：
10.1128/iai.45.1.94-100.1984
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发表时间：
1984-01-01
影响因子：
3.1
通讯作者：
HAYES, SF
中科院分区：
文献类型：
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作者：
BARBOUR, AG;TESSIER, SL;HAYES, SF
两个小鼠单克隆抗体（H6831和H5 TS）不同，在其间接免疫荧光反应时，对14株莱姆病螺旋体进行测试。菌株被两种抗体结合，被单独的H6831或H5 TS结合或不被两者结合。Western印迹和免疫沉淀研究揭示，两种抗体的决定簇与表观亚基MW为λ的丰富蛋白质相关。34，000（34 K范围蛋白质）。以下结果表明，34 K范围的蛋白暴露在螺旋体的表面上：抗体H6831凝集螺旋体;免疫EM显示H6831决定簇与外膜结合;放射性标记的H6831与活生物体结合;蛋白酶有效地从完整细胞中去除34 K范围的蛋白。由于它们在表面上表现出的可变性和暴露性，34 K范围的蛋白质可能有助于莱姆病螺旋体的血清型特异性。
Two mouse monoclonal antibodies (H6831 and H5TS) differed in their indirect immunofluorescence reactivity when tested against 14 strains of Lyme disease spirochetes. Strains were bound by both antibodies, by H6831 or H5TS alone or by neither. Western blot and immunoprecipitation studies revealed that the determinants of both antibodies were associated with abundant proteins with apparent subunit MW of .apprx. 34,000 (34K-range proteins). The following results indicated that the 34K-range proteins were exposed on the surface of the spirochetes: antibody H6831 agglutinated the spirochetes; immune EM showed that the H6831 determinant was associated with the outer membrane; radiolabeled H6831 bound to live organisms; and proteases effectively removed the 34K-range proteins from intact cells. With their demonstrated variability and exposure on the surface, the 34K-range proteins may contribute to the serotype specificity of Lyme disease spirochetes.