Localization of insulin-like growth factor-binding protein-5 messenger ribonucleic acid in rat ovaries during the estrous cycle.
Localization of insulin-like growth factor-binding protein-5 messenger ribonucleic acid in rat ovaries during the estrous cycle.
复制标题
DOI：
10.1210/endo.130.4.1372237
复制
发表时间：
1992-04
期刊：
影响因子：
4.8
通讯作者：
Gregory F. Erickson;Akira Nakatani;Nicholas Ling;Shunichi Shimasaki
中科院分区：
文献类型：
--
作者：
Gregory F. Erickson;Akira Nakatani;Nicholas Ling;Shunichi Shimasaki
为探讨胰岛素样生长因子结合蛋白-5（IGFBP-5）在卵巢生理中的潜在作用，我们采用原位杂交和北方分析法对大鼠动情周期卵巢中IGFBP-5 mRNA进行了定位。通过北方分析，mRNA在周期的所有阶段都是丰富的。检测到两种mRNA，大小分别为6.0和1.8个碱基。两种转录本的相对量在整个周期中发生变化。通过原位杂交，IGFBP-5 mRNA仅在以下几种细胞类型中表达：1）一些闭锁卵泡的颗粒细胞，2）一些次级间质细胞，3）一些黄体，和4）表面上皮。颗粒细胞和次级间质细胞中的信息水平在周期中发生变化。在发情前期1000 h（LH/FSH峰之前），仅在少数卵泡中表达该信息。有趣的是，所有卵泡都是小的闭锁腔前卵泡（200-250微米）。在发情前期2000 h（LH/FSH峰后），IGFBP-5 mRNA更丰富，现在几乎每个闭锁的腔前卵泡显示出强烈的杂交信号。在发情后0200和1000 h，在部分闭锁窦卵泡的颗粒细胞和次级间质细胞中首次出现该mRNA。因此，几乎所有闭锁卵泡，窦前和窦，现在显示IGFBP-5基因表达。与发情前期和发情期相比，发情间期第1天和第2天的杂交信号不太突出，仅在少数闭锁的腔前卵泡中发现。在整个周期中，IGFBP-5 mRNA在一些黄体中明显，但不是特别突出。丰富的IGFBP-5 mRNA在表面上皮中是明显的，并且在周期中没有检测到变化。优势卵泡缺乏IGFBP-5 mRNA。总之，本文提出的第一个证据表明，IGFBP-5基因在成年大鼠卵巢表达。IGFBP基因以细胞特异性方式表达，例如在闭锁颗粒细胞、次级间质细胞、黄体和表面上皮中，并且周期的阶段显著影响信息水平，特别是在发情早晨周围的闭锁颗粒细胞和次级间质细胞中。这些发现表明IGBP-5可能是卵巢生理学的自分泌/旁分泌调节剂，特别是与腔前卵泡闭锁有关。
To investigate the potential role of insulin-like growth factor-binding protein-5 (IGFBP-5) in ovarian physiology, we employed in situ hybridization and Northern analysis to localize IGFBP-5 mRNA in rat ovaries during the estrous cycle. By Northern analysis, the mRNA was abundant at all stages of the cycle. Two species of mRNAs were detected, with sizes of 6.0 and 1.8 kilobases, respectively. The relative amounts of the two transcripts changed throughout the cycle. By in situ hybridization, IGFBP-5 mRNA was expressed in only a few cell types: 1) granulosa cells of some atretic follicles, 2) some secondary interstitial cells, 3) some corpora lutea, and 4) the surface epithelium. The levels of message in both the granulosa and secondary interstitial cells changed over the cycle. At 1000 h on proestrus (before the LH/FSH surge), the message was expressed in only a few follicles. Interestingly, all were small atretic preantral (200-250 microns) follicles. At 2000 h on proestrus (after the LH/FSH surge), the IGFBP-5 mRNA was more abundant; now almost every atretic preantral follicle showed a strong hybridization signal. At 0200 and 1000 h on estrus, the mRNA appeared for the first time in granulosa cells of some atretic antral follicles and in secondary interstitial cells. Hence, virtually all atretic follicles, preantral and antral, now showed IGFBP-5 gene expression. In contrast to that on proestrus and estrus, the hybridization signal on diestrous days 1 and 2 was much less prominent and was found in only a few atretic preantral follicles. Throughout the cycle, IGFBP-5 mRNA was evident in some corpora lutea, but it was not particularly prominent. Abundant IGFBP-5 mRNA was evident in the surface epithelium, and no change was detected over the cycle. Dominant follicles were devoid of IGFBP-5 mRNA. In conclusion, this paper presents the first evidence that the IGFBP-5 gene is expressed in the adult rat ovary. The IGFBP gene is expressed in a cell-specific manner, e.g. in atretic granulosa, secondary interstitial cells, corpora lutea, and the surface epithelium, and the stage of the cycle significantly affected message levels, especially in atretic granulosa and secondary interstitial cells around estrous morning. These findings suggest that IGBP-5 may be an autocrine/paracrine regulator of ovarian physiology, particularly in relation to preantral follicle atresia.