Loss of neoplastic properties in vitro. II. Observations on KB sublines.
Loss of neoplastic properties in vitro. II. Observations on KB sublines.
复制标题
体外肿瘤特性丧失。
作者：
G. Foley;A. Handler;P. M. Lynch;S. Wolman;C. Stulberg;H. Eagle
摘要对从多个不同实验室获得的KB细胞系的10个亚系的染色体数目和异种移植到叙利亚仓鼠颊囊中的情况进行了重新检查。来自其中两个亚系的12个克隆培养物也进行了类似的检查。这些亚系彼此不同，与最初研究的KB细胞系不同，KB细胞系可通过接种约10个细胞进行异种移植。接种细胞数在104个以下的亚系中，仅2株可异种移植，104~106细胞的亚系中有6株可异种移植，即使接种量为106个，仍有2株移植失败。其中两个亚系的克隆衍生品在异种移植能力上也有类似的差异。虽然这些亚系和克隆衍生品的模式数基本相似，从73到80不等，但少数品系的染色体具有足够独特的形态，可以作为标记。然而，在这些微小的核型差异和异种移植能力之间没有明显的相关性。非异种可移植亚系和异种可移植亚系在每细胞胞质蛋白质和胞质核糖核酸(RNA)含量变异模式方面的可能差异仍有待详细研究。
Summary Ten sublines of the KB cell line obtained from a number of different laboratories were reexamined with respect to chromosome number and heterotransplantability to the Syrian hamster cheek pouch. Twelve clonal cultures derived from 2 of these sublines were similarly examined. These sublines differed from one another and from the KB cell line originally studied, which was heterotransplantable with inocula of circa 10 cells. Only 2 of the sublines could be heterotransplanted with inocula of less than 10 4 cells; 6 were heterotransplantable with inocula of 10 4 -10 6 cells; and 2 failed to heterotransplant, even with inocula of 10 6 cells. Similar differences in heterotransplantability were observed among the clonal derivatives of 2 of these sublines. Although the modal numbers of these sublines and clonal derivatives were basically similar, ranging from 73 to 80, a few lines had a chromosome of sufficiently distinctive morphology to serve as a marker. There was, however, no apparent correlation between these minor karyotypic differences and heterotransplantability. Possible differences between nonheterotransplantable and heterotransplantable sublines with respect to the pattern of variability in cytoplasmic protein and cytoplasmic ribonucleic acid (RNA) content per cell remain to be studied in detail.