Genetic diversity of central and peripheral populations of Toona ciliata var. pubescens, an endangered tree species endemic to China
Genetic diversity of central and peripheral populations of Toona ciliata var. pubescens, an endangered tree species endemic to China
复制标题
香椿中部和外围群体的遗传多样性。
DOI：
10.4238/2014.june.17.10
复制
发表时间：
2014-01-01
影响因子：
0.4
通讯作者：
Chen, Y. T.
中科院分区：
文献类型：
--
作者：
Liu, J.;Jiang, J. M.;Chen, Y. T.
本研究的目的是研究红椿中心种群和边缘种群的遗传多样性。毛，以阐明是否中央外周假说适用于这些人群。利用8对多态性SSR引物对9个自然居群的392个个体进行了分析。结果表明，在周边群体中观察到的等位基因数和期望等位基因数均高于中心群体。常见的广泛分布和罕见的本地（RL）等位基因在所有人群中观察到。然而，在5个群体中发现了常见的局部等位基因，仅在3个群体中发现了罕见的广泛分布等位基因。4种等位基因总数在周边群体中均高于中心群体，且RL等位基因数量在周边群体中明显高于中心群体。观察到的和预期的杂合性较高，在周边人群相比，中心人群。周边种群的基因分化系数为0.3045，显著高于中心种群。中心种群间的基因流大于1，而边缘种群间的基因流小于1。这表明，频繁的基因流存在于中心种群之间，而地形和栖息地破碎化阻止了周边种群之间的基因流。Mantel检验表明，T的遗传距离与地理距离之间没有关系。纤毛变种毛
Our objective was to examine the genetic diversity of central and peripheral populations of Toona ciliata var. pubescens, to elucidate whether the central-peripheral hypothesis applies to these populations. We analyzed 392 individuals from nine natural populations using eight pairs of polymorphic SSR primers. The results showed that the mean numbers of observed and expected alleles in peripheral populations were higher than in central populations. Common widespread and rare local (RL) alleles were observed in all populations. However, common local alleles were found in five populations and rare widespread alleles were only found in three. The total numbers of the four types of alleles were higher in peripheral than in central populations, and the quantity of the RL allele was obviously higher in the peripheral populations than in the central populations. Both the observed and expected heterozygosities were higher in peripheral populations compared with the central populations. The coefficient of gene differentiation of the peripheral populations was 0.3045, which was significantly higher than that of the central populations. The gene flow between central populations was greater than one, but less than one between peripheral populations. This indicates that frequent gene flow exists between central populations, while terrain and habitat fragmentation prevent gene flow between peripheral populations. A Mantel test indicated that there was no relationship between genetic and geographical distance of T. ciliata var. pubescens.