HEAT-SHOCK PROTEINS AND THERMOTOLERANCE - A COMPARISON OF INDUCTION KINETICS
HEAT-SHOCK PROTEINS AND THERMOTOLERANCE - A COMPARISON OF INDUCTION KINETICS
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DOI：
10.1259/0007-1285-55-656-579
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发表时间：
1982-01-01
影响因子：
2.6
通讯作者：
JOHNSON, RJ
中科院分区：
文献类型：
--
作者：
SUBJECK, JR;SCIANDRA, JJ;JOHNSON, RJ
假设，初步高热处理后热休克蛋白的表达与随后对第二次热疗的耐热性有关。CHO细胞受照12min，45度。C预处理，然后在37℃下不同时间间隔孵育。C.通过用SDS[十二烷基硫酸钠]聚丙烯酰胺凝胶电泳法测定的35S-Met掺入法，监测某些细胞内蛋白质的合成作为孵育后时间的函数。通过对细胞进行第二次热处理(45度)，同时测量细胞存活率。C/27min)。在68,000，89,000和110,000道尔顿观察到主要的热休克蛋白。在环己胺存在的情况下，这些蛋白质的合成显著减少。总的35S-Met掺入这些蛋白质中与诱导存活抗性(耐热性)有很好的相关性。存活率与每种蛋白质的含量之间可能出现近似的指数关系。这些和其他热休克蛋白在正常培养条件下保持在37度的对照(非热休克)细胞中也存在显著降低的程度。C.热休克蛋白可能与热耐受现象有关。
Hypothetically, the expression of heat shock proteins following a preliminary hyperthermic treatment is responsible for subsequent thermotolerance to a 2nd heat treatment. CHO cells were given a 12 min, 45.degree. C pretreatment and then incubated for varying intervals at 37.degree. C. The synthesis of certain intracellular proteins was monitored as a function of time post-incubation by using 35S-Met incorporation as determined in SDS [sodium dodecyl sulfate] polyacrylamide gel electrophoresis. Cell survival was concurrently measured by challenging the cells with a 2nd heat treatment (45.degree. C/27 min). Major heat shock proteins were observed at 68,000, 89,000 and 110,000 daltons. The synthesis of these proteins was significantly reduced in the presence of cyclohexamide. The total 35S-Met incorporation into these proteins correlated well with the induction of survival resistance (thermotolerance). An approximate exponential relationship between survival and the amount of each of these proteins may occur. These and other heat shock proteins were also present, in a significantly reduced degree, in control (non-heat shocked) cells maintained under normal culture conditions at 37.degree. C. Heat shock proteins may be responsible for the phenomenon of thermotolerance.