Effects of a choline-deficient diet and a hypolipidemic agent on single glutathione S-transferase placental form-positive hepatocytes in rat liver.
Effects of a choline-deficient diet and a hypolipidemic agent on single glutathione S-transferase placental form-positive hepatocytes in rat liver.
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缺乏胆碱的饮食和降血脂剂对大鼠肝脏中单一谷胱甘肽 S-转移酶胎盘型阳性肝细胞的影响。
DOI：
10.1111/j.1349-7006.1990.tb02538.x
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Shinozuka,H
中科院分区：
文献类型：
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作者：
Yokota,K;Singh,U;Shinozuka,H
以胎盘形式的谷胱甘肽转移酶(GST-P)作为致癌物启动的肝细胞标志物，我们研究了胆碱缺乏(CD)饮食和致癌降脂剂BR931如何改变单个GST-P阳性肝细胞的群体。饲喂CS或基础饲料的Fischer雄性大鼠(6-7周龄)的肝脏主要含有单个或双个GST-P阳性的肝细胞。饲喂Cd饲料2-4周后，两个和三个GST-P阳性肝细胞聚集的数量增加。8~12周时，出现由20多个肝细胞组成的GST-P阳性肝细胞的散在灶。饲喂BR931饲料4-8周，与饲喂基础饲料相比，肝脏中单个GST-P阳性肝细胞的数量没有显著变化。提示商业饲料饲养的相对年轻大鼠肝脏中单个GST-P阳性肝细胞可能代表内源性起始细胞。Cd饮食促进内源性启动的细胞形成更大的GST-P阳性细胞聚集体或焦点。
Using the placental form of glutathioneS‐transferase (GST‐P) as a marker of carcinogen‐initiated hepatocytes, we investigated how a choline‐deficient (CD) diet and BR931, a carcinogenic hypolipidemic agent, modify populations of single GST‐P‐positive hepatocytes. The liver of male Fischer rats (6‐7 weeks old) fed a CS or basal diet contained mostly single or double GST‐P‐positive hepatocytes. Feeding a CD diet for 2–4 weeks led to increases in the number of aggregates of two and three GST‐P‐positive hepatocytes. By 8–12 weeks, there was an emergence of discrete foci of GST‐P‐positive hepatocytes consisting of more than 20 hepatocytes. Feeding a BR931 diet for 4–8 weeks resulted in no significant change in the number of single GST‐P‐positive hepatocytes in the liver as compared to feeding a basal diet. It is suggested that single GST‐P‐positive hepatocytes in the liver of relatively young rats maintained on a commercial diet may represent endogenously initiated cells. A CD diet promotes endogenously initiated cells to form larger aggregates or foci of GST‐P‐positive cells.