Aberrant Cortical Activity in Multiple GCaMP6-Expressing Transgenic Mouse Lines.
Aberrant Cortical Activity in Multiple GCaMP6-Expressing Transgenic Mouse Lines.
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DOI：
10.1523/eneuro.0207-17.2017
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发表时间：
2017-09
期刊：
影响因子：
3.4
通讯作者：
Harris KD
中科院分区：
文献类型：
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作者：
Steinmetz NA;Buetfering C;Lecoq J;Lee CR;Peters AJ;Jacobs EAK;Coen P;Ollerenshaw DR;Valley MT;de Vries SEJ;Garrett M;Zhuang J;Groblewski PA;Manavi S;Miles J;White C;Lee E;Griffin F;Larkin JD;Roll K;Cross S;Nguyen TV;Larsen R;Pendergraft J;Daigle T;Tasic B;Thompson CL;Waters J;Olsen S;Margolis DJ;Zeng H;Hausser M;Carandini M;Harris KD
转基因小鼠系是神经科学的宝贵工具，但与任何技术一样，必须小心确保工具本身不会过度影响所研究的系统。在这里，我们报告异常的电活动，类似于发作间期尖峰，并伴随荧光事件在一些基因型的转基因小鼠表达GCaMP 6基因编码的钙传感器。这些癫痫样事件在多个实验室中特别但不限于Emx 1-Cre和Ai 93转基因小鼠中观察到，无论性别如何。事件发生在>0.1 Hz，振幅非常大（>1.0 mV局部场电位，>10% df/f宽场成像信号），并且通常覆盖皮层的大区域。尽管发生了这些事件，但神经元反应和行为的许多特性似乎是正常的，尽管极少数受试者表现出明显的全身性癫痫发作。这种现象的潜在机制尚不清楚，但我们根据不同的观察结果推测可能的原因。我们鼓励研究人员在解释受影响小鼠品系的神经元记录以及考虑研究哪些品系时注意这些活动模式。
Transgenic mouse lines are invaluable tools for neuroscience but, as with any technique, care must be taken to ensure that the tool itself does not unduly affect the system under study. Here we report aberrant electrical activity, similar to interictal spikes, and accompanying fluorescence events in some genotypes of transgenic mice expressing GCaMP6 genetically encoded calcium sensors. These epileptiform events have been observed particularly, but not exclusively, in mice with Emx1-Cre and Ai93 transgenes, of either sex, across multiple laboratories. The events occur at >0.1 Hz, are very large in amplitude (>1.0 mV local field potentials, >10% df/f widefield imaging signals), and typically cover large regions of cortex. Many properties of neuronal responses and behavior seem normal despite these events, although rare subjects exhibit overt generalized seizures. The underlying mechanisms of this phenomenon remain unclear, but we speculate about possible causes on the basis of diverse observations. We encourage researchers to be aware of these activity patterns while interpreting neuronal recordings from affected mouse lines and when considering which lines to study.