Reagentless biomolecular analysis using a molecular pendulum
Reagentless biomolecular analysis using a molecular pendulum
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DOI：
10.1038/s41557-021-00644-y
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发表时间：
2021-03-08
期刊：
影响因子：
21.8
通讯作者：
Kelley, Shana O.
中科院分区：
文献类型：
--
作者：
Das, Jagotamoy;Gomis, Surath;Kelley, Shana O.
能够检测生物流体中分子分析物的无试剂传感器的开发可以在个性化健康监测中实现广泛的应用。然而，目前只有有限的一组分子输入可以使用无试剂传感器检测。在这里，我们报告了一种传感机制，该机制与分析蛋白质是压力，过敏，心血管健康，炎症和癌症的重要生理标志物是兼容的。该传感方法是基于一个倒置的分子摆的运动，表现出场诱导的运输调制的存在下的结合分析物。我们使用附着的报告分子的电子转移动力学来测量传感器的电场介导的运输。使用时间分辨的电化学测量，使我们的传感器的单向运动，结合到我们的传感器复合物的分析物的存在可以连续地跟踪在真实的时间。我们表明，这种传感方法与血液，唾液，尿液，眼泪和汗液的测量兼容，并且传感器可以在活体动物中原位收集数据。
The development of reagentless sensors that can detect molecular analytes in biological fluids could enable a broad range of applications in personalized health monitoring. However, only a limited set of molecular inputs can currently be detected using reagentless sensors. Here, we report a sensing mechanism that is compatible with the analysis of proteins that are important physiological markers of stress, allergy, cardiovascular health, inflammation and cancer. The sensing method is based on the motion of an inverted molecular pendulum that exhibits field-induced transport modulated by the presence of a bound analyte. We measure the sensor's electric field-mediated transport using the electron-transfer kinetics of an attached reporter molecule. Using time-resolved electrochemical measurements that enable unidirectional motion of our sensor, the presence of an analyte bound to our sensor complex can be tracked continuously in real time. We show that this sensing approach is compatible with making measurements in blood, saliva, urine, tears and sweat and that the sensors can collect data in situ in living animals.