Identification of GABAA Receptor Subunits in Rat Retina: Cloning of the Rat GABAA Receptor ρ2‐Subunit cDNA
Identification of GABAA Receptor Subunits in Rat Retina: Cloning of the Rat GABAA Receptor ρ2‐Subunit cDNA
复制标题
大鼠视网膜 GABAA 受体亚基的鉴定：克隆大鼠 GABAA 受体 ρ2 亚基 cDNA
作者：
T. Ogurusu;H. Taira;R. Shingai
摘要：我们通过 PCR 鉴定了大鼠视网膜中的 GABAA 受体亚基。先前描述的跨膜域中配体门控阴离子通道成员之间的高度保守性被用来设计简并有义和反义寡核苷酸。这些寡核苷酸用作 PCR 的引物，应用于大鼠视网膜 cDNA。对 PCR 扩增产生的克隆进行分析，鉴定出 GABAAα1、β1、β3 和 γ2 亚基以及甘氨酸 α1 亚基。此外，还获得了两个与人类 GABAAρ 亚基类密切相关的克隆。分子克隆揭示其中之一是人类 ρ2 亚基的大鼠对应物。 Northern 印迹分析证明了视网膜中 ρ 亚基的 mRNA 表达。这些结果进一步支持了大鼠视网膜中荷包牡丹碱不敏感的 GABA 通道由 ρ 亚基组成的假设。
Abstract: We identified GABAA receptor subunits in rat retina using PCR. The high degree of conservation among previously described members of ligand‐gated anion channels in transmembrane domains was used to design degenerate sense and antisense oligonucleotides. These oligonucleotides were used as primers for PCR, which was applied to the rat retina cDNA. Analysis of clones derived from the PCR amplification identified the GABAAα1, β1, β3, and γ2 subunits and the glycine α1 subunit. In addition, two clones closely related to the human GABAAρ‐subunit class were obtained. Molecular cloning revealed one of them as the rat counterpart of the human ρ2 subunit. Northern blot analysis demonstrated the expression of mRNAs for ρ subunits in retina. These results further support the hypothesis that bicuculline‐insensitive GABA channels in rat retina are comprised of ρ subunits.
DOI：
10.1073/pnas.88.10.4318
发表时间：
1991-05-01
影响因子：
11.1
作者：
POLENZANI, L;WOODWARD, RM;MILEDI, R
通讯作者：
MILEDI, R