3D micromixer for nanoliposome synthesis: a promising advance in high mass productivity
3D micromixer for nanoliposome synthesis: a promising advance in high mass productivity
复制标题
DOI：
10.1039/d1lc00232e
复制
发表时间：
2021-06-07
期刊：
影响因子：
6.1
通讯作者：
de la Torre, Lucimara G.
中科院分区：
文献类型：
--
作者：
Firmino, Priscilla C. O. S.;Vianna, Savio S. V.;de la Torre, Lucimara G.
本文介绍了微流体技术在脂质体大批量生产中的重要突破。我们研究了使用高流速微流体装置（HFR-MD）合成脂质体，该装置具有3d扭曲的横截面微通道，有利于混沌平流。采用简易支架技术制备HFR-MD。脂质体的合成结合了高流量和高浓度脂质的影响，导致高质量生产率（每小时2.27 g脂质），据我们所知，从未仅通过一个微设备记录过。我们评估了流速比（FRR）、总流速（TFR）和脂质浓度对脂质体理化性质的影响。在FRR为1 （50% v/v乙醇），TFR为5 ml min(-1)（可膨胀至10 ml min(-1)）的条件下，HFR-MD脂质体为单分散（0.074），大小约为100 nm。通过实验和计算流体动力学分析，我们证明了混合条件不是控制脂质体合成的唯一参数。采用真空浓缩器进行乙醇脱除，经结构（SAXS）和形态（冷冻透射电镜）分析，处理后无进一步改性。因此，HFR-MD可用于制备纳米脂质体。它是一种大批量生产的创新工具。
This paper addresses an important breakthrough in the high mass production of liposomes by microfluidics technology. We investigated the synthesis of liposomes using a high flow rate microfluidic device (HFR-MD) with a 3D-twisted cross-sectional microchannel to favor chaotic advection. A simple construction scaffold technique was used to manufacture the HFR-MD. The synthesis of liposomes combined the effects of high flow and high concentration of lipids, resulting in high mass productivity (2.27 g of lipid per h) which, to our knowledge, has never been registered by only one microdevice. We assessed the effects of the flow rate ratio (FRR), total flow rate (TFR), and lipid concentration on the liposome physicochemical properties. HFR-MD liposomes were monodisperse (0.074) with a size around 100 nm under the condition of an FRR of 1 (50% v/v ethanol) and TFR of 5 ml min(-1) (expandable to 10 ml min(-1)). We demonstrated that the mixing conditions are not the only parameter controlling liposome synthesis using experimental and computational fluid dynamics analysis. A vacuum concentrator was used for ethanol removal, and there is no further modification after processing in accordance with the structural (SAXS) and morphological (cryo-TEM) analysis. Hence, the HFR-MD can be used to prepare nanoliposomes. It emerges as an innovative tool with high mass production.