Glutamate in the parabrachial nucleus of rats during conditioned taste aversion
Glutamate in the parabrachial nucleus of rats during conditioned taste aversion
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条件性味觉厌恶期间大鼠臂旁核中的谷氨酸
DOI：
10.1016/s0006-8993(00)02986-3
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发表时间：
2000
期刊：
影响因子：
2.9
通讯作者：
J. Křivánek
中科院分区：
文献类型：
--
作者：
E. Bielavská;I. Mikšík;J. Křivánek
采用脑微透析结合高效液相色谱和光谱检测技术，对大鼠条件性味觉厌恶（CTA）获得过程中臂旁核（PBN）细胞外谷氨酸（Glu）的动态变化进行了研究。使用每20分钟取一次的微透析级分来评估单独条件刺激（CS，消耗0.1%糖精）、单独非条件刺激（US，腹膜内注射0.15 M LiCl，2% b.w.饮用水后引起不适）以及CS-US配对。糖精饮用15分钟后，在盐水中的谷氨酸浓度（20 μl/20分钟）达到基线以上的80%，但在下一个部分中恢复到基础值。单独的氯化锂（应用1小时后，15分钟的饮水）增加谷氨酸只有在一些延迟，即在第二和第三后锂馏分分别为90和67%。然而，当氯化锂注射糖精摄入后1小时，谷氨酸浓度显着上升（95%）已经在第一后氯化锂馏分和120%，在第二个。因此，看来，“糖精痕迹”有利于锂的作用，在收购CTA的PBN谷氨酸的细胞外浓度。
Brain microdialysis combined with HPLC and spectroscopic detection was used to monitor extracellular glutamate in the parabrachial nucleus (PBN) of rats during acquisition of a conditioned taste aversion (CTA). Microdialysis fractions taken every 20 min were used to assess the effects of presentation of the conditioned stimulus alone (CS, consumption of 0.1% saccharin), the unconditioned stimulus alone (US, intraperitoneal injection of 0.15 M LiCl, 2% b.w. induced malaise after water drinking) as well as that of CS–US pairing. After 15 min of saccharin drinking, the glutamate concentration in the eluate (20 μl/20 min) reached 80% above the baseline but returned to the basal value in the next fraction. LiCl alone (applied 1 h after 15 min drinking of water) increased glutamate only following some delay, i.e. in the second and third post-lithium fraction by 90 and 67%, respectively. However, when LiCl was injected 1 h after the onset of saccharin intake, the glutamate concentration rose significantly (by 95%) already in the first post-LiCl fraction and by 120% in the second one. It appears, therefore, that the ‘saccharin trace’ facilitates the effect of lithium on extracellular concentration of glutamate in PBN during acquisition of CTA.