Immunopathologic responses to non-lethal sepsis
Immunopathologic responses to non-lethal sepsis
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DOI：
10.1097/00024382-199908000-00005
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发表时间：
1999-08-01
期刊：
影响因子：
3.1
通讯作者：
Remick, DG
中科院分区：
文献类型：
--
作者：
Ebong, SJ;Call, DR;Remick, DG
尽管脓毒症导致显着的发病率和死亡率，但其基本病理学仍不清楚。我们使用盲肠结扎穿刺（CLP）研究了非致死性脓毒症的炎症和生理变化，这是一种由于混合肠道菌群诱发腹膜炎的模型，再现了脓毒症的复杂免疫学。对几组小鼠进行 CLP（25G 针）或假手术，植入微型测量器以连续监测温度和活动，并每天处死小鼠，持续 6 天。 CLP 组出现明显的体温过低（术后 6-13 小时）、活动量（至第 4 天）和体重（至第 3 天）下降，但没有死亡。与假手术动物相比，CLP 治疗小鼠的血液分析显示，血红蛋白、血小板、淋巴细胞、单核细胞和中性粒细胞减少。两组的腹膜中性粒细胞流入量几乎相同。第 1 天，CLP 治疗小鼠的血浆和腹膜 G-CSF、IL-6 以及鼠趋化因子 KC 和 MIP2-α 显着升高。血浆和腹膜 TNF 较低（
Although sepsis causes significant morbidity and mortality, its basic pathology is still not well understood. We investigated the inflammatory and physiologic alterations of non-lethal sepsis using cecal ligation and puncture (CLP), a model that induces peritonitis due to mixed intestinal flora, reproducing the complex immunology of sepsis. Groups of mice were subjected to CLP (25G needle) or sham surgery, had minimitters implanted to continuously monitor temperature and activity, and were sacrificed daily for 6 days. There was significant hypothermia (6-13 hrs post-surgery), and decreases in activity (to day 4) and weight (to day 3) but no mortality in the CLP group. Blood analyses of the CLP-treated mice showed reduced hemoglobin, platelets, lymphocytes, monocytes, and neutrophils, compared to sham animals. Both groups had nearly equivalent neutrophil influx into the peritoneum. Plasma and peritoneal G-CSF, IL-6, as well as the murine chemokines KC and MIP2-alpha were significantly higher in the CLP-treated mice at day 1. Plasma and peritoneal TNF were low (