MICROMANIPULATION STUDIES OF THE MITOTIC APPARATUS IN SAND DOLLAR EGGS
MICROMANIPULATION STUDIES OF THE MITOTIC APPARATUS IN SAND DOLLAR EGGS
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DOI：
10.1002/cm.970100122
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发表时间：
1988-01-01
影响因子：
--
通讯作者：
NAKANO, Y
中科院分区：
文献类型：
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作者：
HIRAMOTO, Y;NAKANO, Y
本文研究了沙枣受精卵和卵裂球有丝分裂纺锤体的力学特性以及有丝分裂器的各种操作对染色体运动和纺锤体伸长的影响。基于用一对微针对锭子进行机械拉伸和压缩的结果以及微注射到锭子中的油滴的行为，得出的结论是，锭子的赤道区域比半锭子区域在机械上更弱。减数分裂后期染色体的移动发生在去掉紫苑或将紫苑与其极端分离的纺锤体和去掉带间区的纺锤体中。这一事实表明，染色体在后期通过在半纺锤体的动粒附近产生的力向极移动。由于紫苑与纺锤体的分离或去除对纺锤体后期伸长和紫苑行为的影响，可以得出结论，纺锤体后期伸长是由附着在纺锤体末端的紫苑产生的拉力引起的。
Mechanical properties of the mitotic spindle and the effects of various operations of the mitotic apparatus on the chromosome movement and spindle elongation were investigated in fertilized eggs and blastomeres of the sand dollar,Clypeaster japonicus. On the basis of results with mechanical stretching and compression of the spindle with a pair of microneedles and the behavior of an oil drop microinjected into the spindle, it was concluded that the equatorial region of the spindle is mechanically weaker than the half‐spindle region. Anaphase chromosome movement occurred in the spindle from which an aster had been removed or separated with its polar end and in the spindle in which the interzonal region had been removed. This fact indicates that chromosomes move poleward in anaphase by forces generated near the kinetochores in the half‐spindle. Because of the effects of separation or removal of an aster from the spindle on the spindle elongation in anaphase and the behavior of the aster, it was concluded that the spindle elongation in anaphase is caused by pulling forces generated by asters attached to the ends of the spindle.