High temperature elevates carotenoid accumulation of banana fruit via upregulation of MaEIL9 module.
High temperature elevates carotenoid accumulation of banana fruit via upregulation of MaEIL9 module.
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DOI：
10.1016/j.foodchem.2023.135602
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发表时间：
2023-01
期刊：
影响因子：
8.8
通讯作者：
Li-sha Zhu;Wei Shan;Danling Cai;Zengxiang Lin;Chaojie Wu;Wei Wei-Wei;Ying-ying Yang;Wangjin Lu;Jianye Chen;Xin-guo Su;Jianfei Kuang
中科院分区：
文献类型：
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作者：
Li-sha Zhu;Wei Shan;Danling Cai;Zengxiang Lin;Chaojie Wu;Wei Wei-Wei;Ying-ying Yang;Wangjin Lu;Jianye Chen;Xin-guo Su;Jianfei Kuang
香蕉是类胡萝卜素的良好来源，类胡萝卜素是具有健康有益特性的生物活性代谢产物。然而，香蕉果实中类胡萝卜素积累的分子机制在很大程度上还不清楚。在这项研究中，我们发现，高温升高类胡萝卜素生产香蕉果肉，这可能是由于上调的一个子集的类胡萝卜素基因，以及类胡萝卜素生物合成regulatorMaSPL16。此外，还鉴定了一个乙烯信号转导元件MaEIL9，其转录和蛋白质含量也受到高温的诱导。此外，MaEIL9通过直接靶向MaDXR1、MaPDS1、MaZDS1和MaSPL16的启动子而正向调节其转录。MaEIL9在番茄果实中的过表达显著增加了类胡萝卜素形成基因的表达，提高了类胡萝卜素含量。重要的是，瞬时沉默香蕉果实中的MaEIL 9减弱了高温引起的类胡萝卜素的产生。综上所述，这些结果表明高温诱导香蕉果实中类胡萝卜素的产生，至少部分是通过MaEIL9介导的MaDXR1、MaPDS1、MaZDS1和MaSPL16表达的激活。
Banana is a good source of carotenoids, which are bioactive metabolites with health beneficial properties for human. However, the molecular mechanism of carotenoid accumulation in banana fruit is largely unclear. In this study, we found that high temperature elevated carotenoid production in banana pulp, which is presumably due to upregulation of a subset of carotenogenic genes as well as a carotenoid biosynthesis regulatorMaSPL16. Moreover, an ethylene signaling component MaEIL9 was identified, whose transcript and protein contents were also induced by high temperature. In addition, MaEIL9 positively regulates transcription ofMaDXR1,MaPDS1,MaZDS1andMaSPL16through directly targeting their promoters. Overexpression ofMaEIL9in tomato fruit substantially increased the expression of carotenoid formation genes and elevated carotenoid content. Importantly, transiently silencingMaEIL9in banana fruit weakened carotenoid production caused by high temperature. Taken together, these results indicate that high temperature induces carotenoid production in banana fruit, at least in part, through MaEIL9-mediated activation ofMaDXR1,MaPDS1,MaZDS1andMaSPL16expression.