Rapid and sensitive bioassay to study signals between root exudates and arbuscular mycorrhizal fungi
Rapid and sensitive bioassay to study signals between root exudates and arbuscular mycorrhizal fungi
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DOI：
10.1023/a:1008938527757
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发表时间：
1999-12-01
影响因子：
--
通讯作者：
Douds, DD
中科院分区：
文献类型：
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作者：
Nagahashi, G;Douds, DD
为了检测植物化学信号对AM真菌萌发孢子菌丝生长的影响，建立了一种灵敏的生物测定方法。该测定可用于测试宿主根分泌物，以及颗粒级分（根冠边缘细胞和根粘液），其影响AM真菌生长的能力。尽管生物测定的结果通常在16至24 h后确定，但由各种寄主根组分诱导的菌丝分支最早可在4 h检测到。观察到的分支模式类型具有剂量依赖性。
A sensitive bioassay was developed to provide a way to detect chemical signals from host plants which induce changes in hyphal growth patterns of germinated spores of arbuscular mycorrhizal (AM) fungi. The assay can be used to test host root exudates, as well as particulate fractions (root cap border cells and root mucilage), for their ability to affect AM fungal growth. Hyphal branching, induced by various host root components, can be detected as early as 4 h although results of the bioassay were usually determined after 16 to 24 h. The type of branching pattern observed was dose-dependent.