Effects of anticoagulants and storage conditions on clinical oxylipid levels in human plasma
Effects of anticoagulants and storage conditions on clinical oxylipid levels in human plasma
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DOI：
10.1016/j.bbalip.2018.10.003
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发表时间：
2018-12-01
影响因子：
4.8
通讯作者：
Giera, Martin
中科院分区：
文献类型：
--
作者：
Jonasdottir, Hulda S.;Brouwers, Hilde;Giera, Martin
代谢组学和脂类组学对个性化医疗保健具有重要意义。特别是生物活性脂类的分析对于更好地了解各种疾病具有重要意义。在临床程序中，血液样本被广泛用于诊断和研究目的。因此，为了保证样本的完整性和相关的研究结果，血液采集和储存的标准化和有效性程序是强制性的。我们研究了不同的血浆储存条件及其对血浆脂肪酸和氧脂水平的影响。我们的数据清楚地表明了储存条件对血浆脂肪组学分析的重要性。在很低的温度(80摄氏度)下储存和采样后直接添加甲醇是避免体外合成氧基脂的最重要措施。此外，我们确定了关键分析物在某些储存条件下会受到影响。最后，我们进行了手性分析，发现即使在-20℃下，可能的残留酶活性也是氧基脂体外形成的贡献者之一。
Metabolomics and lipidomics are of fundamental importance to personalized healthcare. Particularly the analysis of bioactive lipids is of relevance to a better understanding of various diseases. Within clinical routines, blood derived samples are widely used for diagnostic and research purposes. Hence, standardized and validated procedures for blood collection and storage are mandatory, in order to guarantee sample integrity and relevant study outcomes. We here investigated different plasma storage conditions and their effect on plasma fatty acid and oxylipid levels. Our data clearly indicate the importance of storage conditions for plasma lipidomic analysis. Storage at very low temperature (80 degrees C) and the addition of methanol directly after sampling are the most important measures to avoid ex vivo synthesis of oxylipids. Furthermore, we identified critical analytes being affected under certain storage conditions. Finally, we carried out chiral analysis and found possible residual enzymatic activity to be one of the contributors to the ex vivo formation of oxylipids even at - 20 degrees C.