Schineria larvae gen. nov., sp nov., isolated from the 1st and 2nd larval stages of Wohlfahrtia magnifica (Diptera: Sarcophagidae)
Schineria larvae gen. nov., sp nov., isolated from the 1st and 2nd larval stages of Wohlfahrtia magnifica (Diptera: Sarcophagidae)
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DOI：
10.1099/00207713-51-2-401
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发表时间：
2001-03-01
影响因子：
2.8
通讯作者：
Márialigeti, K
中科院分区：
文献类型：
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作者：
Tóth, E;Kovács, G;Márialigeti, K
从专性寄生蝇Wohlfahrtiamagnifica（双翅目：麻蝇科）的蝇幼虫中分离到4株细菌菌株。这些菌株通过多相方法表征，并且代表γ-变形菌门的一个新谱系，因为它们最近的亲缘关系是苛养Xylellafastidiosa（87.1%16SrDNA相似性）。这4个菌株在16 SrDNA水平上是完全相同的，DNA-DNA杂交的相似性为97.8- 102.5%，生理生化特性相似，但对单一碳源的利用不同。所有产生几丁质酶，他们是专性好氧：没有生长被检测到在厌氧条件下，即使在存在的NO;作为终端电子受体.它们的主要呼吸醌是Q-8，DNA的G+C含量为42mol%。它们的细胞膜含有磷脂酰甘油、二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰丝氨酸和两种未知的极性脂质。其主要脂肪酸为C（18：1）、C（16：0）和C（14：0）。为了容纳这些细菌，一个新的属，Schineria gen，nov.，模式种Schineria larvae sp，nov.，本文提出
Four bacterial strains were isolated from the fly larvae of an obligate parasitic fly, Wohlfahrtia magnifica (Diptera: Sarcophagidae), These isolates were characterized by a polyphasic approach and represent a new lineage of gamma -Proteobacteria as their closest relative is Xylella fastidiosa (87.1% 16S rDNA similarity). The four strains are identical at the 16S rDNA level, the level of similarity between them, based on DNA-DNA hybridization, is high (97.8-102.5 %) and they are similar in their physiological and biochemical characteristics, although they differ in their utilization of different sole carbon sources. All produce chitinase, They are obligately aerobic: no growth is detected under anaerobic conditions, even in the presence of NO; as terminal electron acceptor. Their predominant respiratory quinone is Q-8, The G+C content of their DNA is 42 mol%. Their cell membrane contains phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine and two unknown polar lipids. Their main fatty acids are C(18:1), C(16:0) and C(14:0). To accommodate these bacteria, a new genus, Schineria gen, nov., with the type species Schineria larvae sp, nov., is proposed.