Specificity of opsonic antibodies to enhance phagocytosis of Pseudomonas aeruginosa by human alveolar macrophages.
Specificity of opsonic antibodies to enhance phagocytosis of Pseudomonas aeruginosa by human alveolar macrophages.
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调理性抗体增强人肺泡巨噬细胞对铜绿假单胞菌吞噬作用的特异性。
DOI：
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发表时间：
1975
影响因子：
15.9
通讯作者：
H H Newball
中科院分区：
文献类型：
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作者：
H. Reynolds;J. A. Kazmierowski;H H Newball
这些研究比较了特异性分泌IgA （sIgA）和IgG抗体促进人肺泡巨噬细胞吞噬活的铜绿假单胞菌的能力。通过正常成年吸烟者和非吸烟者的肺灌洗获得巨噬细胞，并保持体外细胞单层。免疫sIgA和IgG凝集抗体被证明可以包被和调理活菌，而类似的非免疫免疫球蛋白制剂则没有。当肺泡巨噬细胞被活化的14c假单胞菌攻击时，igg反应的细菌比siga活化的生物更容易被摄入和杀死。吸烟者和非吸烟者巨噬细胞的吞噬反应无显著差异。虽然在呼吸道分泌物中可以发现sIgA和IgG抗体，并且两者在肺宿主防御中无疑是重要的，但IgG抗体在增强体外培养的肺泡巨噬细胞对假单胞菌的摄取方面更有优势。对于某些细菌感染，它可能是更重要的呼吸道抗体。
These studies compared the ability of specific secretory IgA (sIgA) and IgG antibodies to promote phagocytosis of viable pseudomonas aeruginosa by human alveolar macrophages. Macrophages were obtained by lung lavage of normal adult smoker and nonsmoker volunteers and were maintained as in vitro cell monolayers. Both immune sIgA and IgG agglutinating antibodies were demonstrated to coat and opsonize viable bacteria, whereas similar nonimmune immunoglobulin preparations did not. When alveolar macrophages were challenged with viable opsonized 14C-labeled Pseudomonas IgG-reacted bacteria were ingested better and killed more readily than sIgA-opsonized organisms. Phagocytic responses were not significantly different between macrophages obtained from smokers and nonsmokers. Although sIgA and IgG antibodies can be found in respiratory secretions and both are undoubtedly important in pulmonary host defense, IgG opsonic antibody was superior in enhancing the uptake of Pseudomonas by in vitro-cultured alveolar macrophages. It may be the more important respiratory antibody for certain bacterial infections.