High specificity of quantitative methylation-specific PCR analysis for MGMT promoter hypermethylation detection in gliomas.
High specificity of quantitative methylation-specific PCR analysis for MGMT promoter hypermethylation detection in gliomas.
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DOI：
10.1155/2009/531692
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发表时间：
2009
影响因子：
--
通讯作者：
Fazio VM
中科院分区：
文献类型：
--
作者：
Parrella P;la Torre A;Copetti M;Valori VM;Barbano R;Notarangelo A;Bisceglia M;Gallo AP;Balsamo T;Poeta ML;Carella M;Catapano D;Parisi S;Dallapiccola B;Maiello E;D'Angelo V;Fazio VM
采用实时定量甲基化特异性PCR（QMSP）分析了28例正常脑组织和50例胶质瘤标本。将该分析的数据与MSP在相同样品上获得的结果进行比较。QMSP分析表明，与正常脑组织相比，肿瘤样品中甲基化水平（P = .000009 Mann Whitney检验）和频率（P = .0000007，Z检验）两者存在统计学显著差异。虽然QMSP和MSP的敏感性相似，但QMSP分析的特异性（93%; CI 95%：84%-100%）显著高于MSP（64%; 95%CI：46%-82%）。我们的研究结果表明，QMSP分析可能是一个强大的工具，以确定胶质瘤患者，将受益于烷化剂化疗。
Normal brain tissue from 28 individuals and 50 glioma samples were analyzed by real-time Quantitative Methylation-Specific PCR (QMSP). Data from this analysis were compared with results obtained on the same samples by MSP. QMSP analysis demonstrated a statistically significant difference in both methylation level (P = .000009 Mann Whitney Test) and frequencies (P = .0000007, Z-test) in tumour samples as compared with normal brain tissues. Although QMSP and MSP showed similar sensitivity, the specificity of QMSP analysis was significantly higher (93%; CI95%: 84%–100%) as compared with MSP (64%; 95%CI: 46%–82%). Our results suggest that QMSP analysis may represent a powerful tool to identify glioma patients that will benefit from alkylating agents chemotherapy.
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DOI：
10.1073/pnas.93.18.9821
发表时间：
1996-09-03
影响因子：
11.1
作者：
Herman, JG;Graff, JR;Baylin, SB
通讯作者：
Baylin, SB
影响因子：
3.5
作者：
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作者：
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通讯作者：
Waha, Andreas
影响因子：
158.5
作者：
Hegi, ME;Diserens, A;Stupp, R
通讯作者：
Stupp, R