THE EFFICACY OF INTRAPERITONEAL PANCREATIC-ISLET ISOGRAFTS IN THE REVERSAL OF DIABETES IN RATS
THE EFFICACY OF INTRAPERITONEAL PANCREATIC-ISLET ISOGRAFTS IN THE REVERSAL OF DIABETES IN RATS
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DOI：
10.1097/00007890-199111000-00004
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发表时间：
1991-11-01
期刊：
影响因子：
6.2
通讯作者：
VANSCHILFGAARDE, R
中科院分区：
文献类型：
--
作者：
FRITSCHY, WM;VANSTRAATEN, JFM;VANSCHILFGAARDE, R
腹膜腔再次引起人们对胰岛移植的兴趣，因为它是免疫隔离胰岛移植的首选部位。在这项研究中，我们研究了链脲佐菌素糖尿病大鼠自由腹膜内同基因移植后恢复正常血糖所需的最小胰岛移植物体积。此外，通过测量葡萄糖和胰岛素对静脉葡萄糖负荷和自发摄入碳水化合物丰富的膳食的反应来测试移植物的功能。A组8 0~10 0，B组4 0~5 0，C组2 0~2 3亩L，1亩L胰岛约30 0个。A组10只大鼠和B组9只大鼠中有7只在移植后至少6个月内血糖正常，血糖水平与正常对照组无显著差异。C组9只动物中只有3只血糖正常，且从未超过3个月。A组和B组大鼠对IVGTT的胰岛素反应与移植胰岛体积成正比，且均显著低于正常对照组。对测试餐的胰岛素反应显示出类似的趋势，这被发现与缺乏吸收前期胰岛素分泌有关。A组和B组大鼠餐后最大血糖水平分别比正常对照组高0.8和1.5 mm。我们的结论是，要逆转链脲佐菌素糖尿病大鼠的血糖，至少需要移植4.0-5.0目的L胰岛组织，而移植8.0-10.0目的L胰岛的大鼠的血糖和胰岛素水平并不完全正常化。提示糖耐量受损是由于移植的胰岛组织的β细胞质量不足，缺乏副交感神经支配所致。
The peritoneal cavity is of renewed interest for pancreatic islet transplantation, since it is the preferable site for transplantation of immunoisolated islets. In this study we investigated the minimum islet graft volume needed to restore normoglycemia after free intraperitoneal isogenic transplantation in streptozotocin diabetic rats. Furthermore, graft function was tested by measuring glucose and insulin response to an intravenous glucose load and spontaneously ingested carbohydrate-rich meal. Three graft volumes were used: 8.0-10.0 (group A); 4.0-5.0 (group B); and 2.0-2.3-mu-l (group C); 1-mu-l contained about 300 islets. All 10 rats in group A and 7 out of 9 rats in group B became normoglycemic for at least 6 months posttransplant, with blood glucose levels not significantly different from normal control animals. Only 3 out of 9 animals in group C became normoglycemic and never for longer than 3 months. The insulin responses to IVGTT in group A and group B were proportional to the grafted islet volume and always significantly lower than those of normal control rats. The insulin response to the test meal showed a similar tendency, which was found to be associated with the absence of preabsorptive insulin secretion. Maximum postprandial blood glucose levels in group A and group B were 0.8 and 1.5 mM higher than in normal control rats. We conclude that intraperitoneal transplantation of at least 4.0-5.0-mu-l islet tissue is needed to reverse blood glucose in streptozotocin diabetic rats, and that glucose and insulin levels on IVGTT and test meal in rats with islet grafts of 8.0-10.0-mu-l are not completely normalized. It is suggested that the impaired glucose tolerance is due to an insufficient beta-cell mass and a lack of parasympathetic innervation of the transplanted islet tissue.