Enrichment and transplantation of spermatogonial stem cells.
Enrichment and transplantation of spermatogonial stem cells.
复制标题
DOI：
10.1046/j.1365-2605.2000.00025.x
复制
发表时间：
2000-04
影响因子：
--
通讯作者：
T. Shinohara;R. Brinster
中科院分区：
文献类型：
--
作者：
T. Shinohara;R. Brinster
精子发生是一个复杂的、高度组织化的过程，起源于精原干细胞。由于干细胞非常少，并且只能根据其功能来定义，因此这些细胞的鉴定和分离非常困难。通过使用精原细胞移植试验系统，我们已经确定了α 6-和β 1-整合素表达的干细胞，这些抗原分离的细胞显着富集干细胞。这是首次证实精原干细胞相关抗原。对两种不育小鼠模型Steel/SteelDickie（Sl/Sld）和实验性隐睾的分析表明，Sl/Sld睾丸中干细胞的数量减少。而隐睾的干细胞非常丰富，每200个细胞中就有一个是干细胞。这些技术将为精原干细胞的进一步纯化和鉴定提供一个重要的起点。
Spermatogenesis is a complex, highly organized process originated from stem cell spermatogonia. Because there are very few stem cells and they can only be defined by their function, the identification and isolation of these cells has been very difficult. By using a spermatogonial transplantation assay system, we have identified alpha6-and beta1-integrin expression on stem cells, and cells isolated with these antigens were significantly enriched in stem cells. This is the first demonstration of spermatogonial stem cell-associated antigens. Analysis of two infertile mouse models, Steel/SteelDickie (Sl/Sld) and experimental cryptorchidism, showed that the number of stem cells is reduced in Sl/Sld testis. Whereas cryptorchid testes are greatly enriched for stem cells, and one in 200 cells is a stem cell. These techniques will provide an important starting point for further purification and characterization of spermatogonial stem cells.