Analysis of serum amyloid A1 exon 4 polymorphism in Japanese population
Analysis of serum amyloid A1 exon 4 polymorphism in Japanese population
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日本人群血清淀粉样蛋白A1外显子4多态性分析
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
Toshiyuki Yamada
中科院分区：
文献类型：
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作者：
Toshiyuki Yamada
本文分析了编码人血清淀粉样蛋白A1（SAA 1）氨基酸72位的三联密码子中的核苷酸变异，以鉴定编码SAA 1.2的基因组序列，并进一步表征日本人群中SAA 1等位基因的频率。通过PCR扩增SAA 1外显子4并用Nco 1处理。对来自Nco I位点杂合个体的基因组DNA的PCR产物进行测序，发现在第72位存在GGT（Gly）和GAT（Asp）。52Asp的等位基因显示编码52Ala和57Val的外显子3多态性。因此，该等位基因应被鉴定为SAA1.2。具有72 Gly的等位基因为52 Val和57 Ala（SAA1.1）或52 Ala和57 Ala（SAA1.3）或52 Ala和57 Val（SAA1.5）。在321名日本受试者中，SAA 1等位基因的频率分别为0.310、0.012、0. 347和0.330。未评价SAA. 4等位基因的存在。
Nucleotide variation in the triplet codon coding for amino acid position 72 of human serum amyloid A1 (SAA1), which was suggested by amino acid sequence analysis, was analyzed here in order to identify the genomic sequences coding SAA1.2 and to characterize further the SAA1 allele frequency in a Japanese population. The SAA1 exon 4 was amplified by PCR and treated with Nco 1. Sequencing of PCR products from genomic DNA of individuals who were heterozygous for the Nco I site revealed GGT(Gly) and GAT (Asp) at the position 72. The allele having 52Asp showed the exon 3 polymorphism coding 52Ala and 57Val. This allele should thus be identified as SAA1.2. Alleles with 72Gly were either 52Val and 57Ala (SAA1.1) or 52Ala and 57Ala (SAA1.3) or 52Ala and 57Val(SAA1.5). The frequency of SAA1 alleles in the 321 Japanese subjects was 0.310, 0.012, 0. 347 and 0.330 for each SAA allele of 1.1, 1.2, 1.3 and 1.5, respectively. The presence of the SAA.4 allele was not evaluated.
DOI：
10.1016/0925-4439(94)00076-3
发表时间：
1995-01-25
影响因子：
6.2
作者：
LIEPNIEKS, JJ;KLUVEBECKERMAN, B;BENSON, MD
通讯作者：
BENSON, MD