Increased glutathione and glutathione peroxidase in lungs of individuals with chronic beryllium disease
Increased glutathione and glutathione peroxidase in lungs of individuals with chronic beryllium disease
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DOI：
10.1164/ajrccm.159.6.9810044
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发表时间：
1999-06-01
影响因子：
24.7
通讯作者：
Erzurum, SC
中科院分区：
文献类型：
--
作者：
Comhair, SAA;Lewis, MJ;Erzurum, SC
活性氧（ROS）是慢性组织损伤和纤维化的介质。内源性抗氧化剂可能会增加对氧化剂的反应，减少组织损伤。我们研究了肺对ROS慢性释放的抗氧化反应，如慢性铍病（CBD）的免疫特异性肉芽肿性炎症，并将其与健康对照组和暴露于香烟烟雾的个体进行了比较。抗氧化剂超氧化物歧化酶（SOD），过氧化氢酶，谷胱甘肽过氧化物酶（GPx），谷胱甘肽（CSH）定量肺上皮衬里液（ELF）和血清从对照组（n = 10），吸烟者（n = 8），和个人与CBD（n = 9）。与对照组和吸烟者相比，CBD受试者ELF中GPx活性和细胞外GPx（eGPx）蛋白增加（ELF中eGPx：对照组，1.3 ± 0.2 μ g/ml，吸烟者，1.9 ± 0.3 μ g/ml，CBD，3.8 ± 0.8 μ g/ml; p = 0.002; GPx U/ml ELF，对照组1.4 +/- 0.3，吸烟者1.8 +/- 0.4，CBD，4.5 +/- 1，p = 0.02）。吸烟者ELF中CSH含量高于对照组，但CBD患者ELF中CSH含量明显高于对照组和吸烟者（p < 0.001）。GSH的增加与eGPx相关，表明这些抗氧化剂具有相似的诱导机制。因此，谷胱甘肽抗氧化系统的协同增强发生在肉芽肿性肺炎中。
Reactive oxygen species (ROS) are mediators of chronic tissue damage and fibrosis. Endogenous antioxidants may increase in response to oxidants and reduce tissue injury. We investigated the antioxidant response of the lungs to the chronic release of ROS, as occurs in the immune-specific granulomatous inflammation of chronic beryllium disease (CBD), and compared it with that in healthy controls and individuals exposed to cigarette smoke. The antioxidants superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), and glutathione (CSH) were quantitated in lung epithelial lining fluid (ELF) and serum from control subjects (n = 10), cigarette smokers (n = 8), and individuals with CBD (n = 9). GPx activity and extracellular GPx (eGPx) protein were increased in the ELF of subjects with CBD in comparison with that of control subjects and smokers (eGPx in ELF: controls, 1.3 +/- 0.2 mu g/ml, smokers, 1.9 +/- 0.3 mu g/ml, CBD, 3.8 +/- 0.8 mu g/ml; p = 0.002; GPx U/ml ELF, controls 1.4 +/- 0.3, smokers 1.8 +/- 0.4, CBD, 4.5 +/- 1, p = 0.02). Smokers' ELF had higher levels of CSH than that of controls, but CBD patients' ELF contained much more CSH than that of either controls or smokers (p < 0.001). Increases in GSH were correlated with eGPx, indicating similar inducing mechanisms for these antioxidants. Thus, coordinate augmentation of the glutathione antioxidant system occurs in granulomatous lung inflammation.