Comparative analysis of hematopoietic growth factors released by stromal cells from normal donors or transplanted patients.
Comparative analysis of hematopoietic growth factors released by stromal cells from normal donors or transplanted patients.
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DOI：
10.1182/blood.v75.1.305.305
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发表时间：
1990
期刊：
影响因子：
20.3
通讯作者：
A. Migliaccio;G. Migliaccio;G. Johnson;J. Adamson;B. Torok-Storb
中科院分区：
文献类型：
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作者：
A. Migliaccio;G. Migliaccio;G. Johnson;J. Adamson;B. Torok-Storb
我们比较了来自九名正常受试者和九名骨髓移植后患者的基质细胞在血清剥夺条件下释放的红细胞破裂促进活性（BPA）和集落刺激活性（CSA）。 BPA 和 CSA 是根据条件培养基 (CM) 在非贴壁骨髓细胞的去血清培养物中刺激红细胞爆发和粒细胞/巨噬细胞 (GM) 集落形成的能力来定义的。在研究期间，六名患者（A 组）未能成功建立或维持同种异体移植。其余三人（B 组）没有遇到植入问题。所有基质细胞培养物的 CM 均含有可检测水平的 BPA。将 CM 与抗 GM 集落刺激因子 (GM-CSF) 单克隆抗体 (MoAb) 预孵育，但不与兔抗白细胞介素 3 (IL-3) 血清预孵育，可使 BPA 平均降低 94%。来自正常和 B 组基质细胞培养物的 CM 含有可检测到的 CSA，其水平与通过特定生物测定检测到的粒细胞 CSF (G-CSF) 的量相关。在由骨髓功能较差的移植患者的基质细胞调节的培养基中未检测到 G-CSF。这些结果表明，来自正常受试者和骨髓功能良好的移植患者的基质细胞的CM同时含有GM-CSF和G-CSF，而来自骨髓功能差的移植患者的基质细胞的CM仅含有可检测水平的GM-CSF。这些基质细胞产生 G-CSF 的能力降低与 CM 维持 GM 集落形成的能力降低有关，并且可能与这些患者无法维持体内中性粒细胞计数有关。
We compared the erythroid burst-promoting activity (BPA) and colony-stimulating activity (CSA) released under serum-deprived conditions by stromal cells derived from nine normal subjects and from nine patients after bone marrow transplantation. BPA and CSA were defined according to the capacity of the conditioned media (CM) to stimulate formation of erythroid bursts and granulocyte/macrophage (GM) colonies in serum-deprived cultures of nonadherent marrow cells. Six patients (group A) failed to establish or maintain successful allografts during the study. The remaining three (group B) did not experience problems with engraftment. CM from all stromal cell cultures contained detectable levels of BPA. Preincubation of the CM with an anti-GM colony-stimulating factor (GM-CSF) monoclonal antibody (MoAb), but not with a rabbit anti-interleukin-3 (IL-3) serum, reduced BPA by an average of 94%. CM from normal and group B stromal cell cultures contained detectable CSA, and the levels correlated with the amounts of granulocyte-CSF (G-CSF) detected by a specific bioassay. G-CSF was not detectable in medium conditioned by stromal cells from transplanted patients with poor marrow function. These results indicate that CM from stromal cells from normal subjects and transplanted patients with good marrow function contain both GM-CSF and G-CSF, while CM from stromal cells from transplanted patients with poor marrow function contain detectable levels of GM-CSF only. The reduced capacity of these stromal cells to produce G-CSF is associated with a reduced capacity of the CM to sustain GM colony formation and may be associated with the inability of these patients to sustain their neutrophil counts in vivo.