SELECTED ION MONITORING METHOD FOR DETERMINATION OF NICOTINE, COTININE AND DEUTERIUM-LABELED ANALOGS - ABSENCE OF AN ISOTOPE EFFECT IN THE CLEARANCE OF (S)-NICOTINE-3',3'-D2 IN HUMANS
SELECTED ION MONITORING METHOD FOR DETERMINATION OF NICOTINE, COTININE AND DEUTERIUM-LABELED ANALOGS - ABSENCE OF AN ISOTOPE EFFECT IN THE CLEARANCE OF (S)-NICOTINE-3',3'-D2 IN HUMANS
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DOI：
10.1002/bms.1200200503
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发表时间：
1991-05-01
期刊：
影响因子：
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通讯作者：
BENOWITZ, NL
中科院分区：
文献类型：
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作者：
JACOB, P;YU, L;BENOWITZ, NL
开发了一种同时测定人血浆中尼古丁、其代谢物可替宁以及稳定同位素标记类似物尼古丁-3',3'-d2 和可替宁-4',4'-d2 的方法。 该方法利用毛细管柱气相色谱法并通过电子轰击质谱法和选择离子监测进行检测。 灵敏度足以测定浓度低至 1 ng ml-1 的尼古丁和尼古丁-d2，以及浓度低至 10 ng ml-1 的可替宁和可替宁-d2，具有良好的精密度和准确度。 该方法已用于比较 (S)-尼古丁-3',3'-d2 与天然尼古丁在人类受试者中的消除动力学。 尼古丁-3',3'-d2 的总清除率实际上与天然尼古丁的总清除率相同，这验证了氘标记类似物在尼古丁代谢处置的定量研究中的使用。
A method for simultaneous determination of nicotine, its metabolite cotinine, and the stable isotope-labeled analogs nicotine-3',3'-d2 and cotinine-4',4'-d2 in human plasma has been developed. The method utilizes capillary column gas chromatography with detection by electron impact mass spectrometry and selected ion monitoring. Sensitivity is adequate for determination of nicotine and nicotine-d2 at concentrations as low as 1 ng ml-1, and cotinine and cotinine-d2 at concentrations as low as 10 ng ml-1 with good precision and accuracy. The method has been used to compare the elimination kinetics of (S)-nicotine-3',3'-d2 with natural nicotine in human subjects. Total clearance of nicotine-3',3'-d2 was virtually identical to the total clearance of natural nicotine, which validates the use of the deuterium-labeled analog in quantitative studies of nicotine metabolic disposition.