Effect of addition of a probiotic micro-organism to broiler diet on intestinal mucosal architecture and electrophysiological parameters
Effect of addition of a probiotic micro-organism to broiler diet on intestinal mucosal architecture and electrophysiological parameters
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DOI：
10.1111/j.1439-0396.2009.00933.x
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发表时间：
2010-08-01
影响因子：
2.7
通讯作者：
Boehm, J.
中科院分区：
文献类型：
--
作者：
Awad, W. A.;Ghareeb, K.;Boehm, J.
益生菌可能是解决近期饲料中禁用抗菌生长促进剂影响的解决方案之一。然而，益生菌的作用方式仍然没有完全了解。因此，评价益生菌（微生物饲料添加剂）至关重要。本研究旨在探讨新型微生物饲料添加剂（唾液乳杆菌和罗伊氏乳杆菌）在肉鸡营养中的效果。在肉鸡日粮中添加乳酸杆菌可提高肉鸡的体重和日增重。此外，乳酸菌饲料添加剂影响小肠绒毛的组织形态学测量。乳酸杆菌的添加增加了绒毛高度（VH）/隐窝深度比（p < 0.05），并且十二指肠中的VH在数值上增加。与对照组相比，十二指肠隐窝深度未受影响（p > 0.05），而回肠隐窝深度则因饲料中添加乳酸杆菌而降低。在喂养期结束时，在分离的肠粘膜中测量基础和葡萄糖刺激的短路电流（Isc）和电组织电导率以表征肠的电特性。相对于乳酸杆菌益生菌组中的基础值，在Ussing室中的粘膜侧添加葡萄糖在空肠和结肠中均产生Isc的显著增加（p = 0.001）。益生菌组在空肠中的Isc的这种增加相当于基础值的约两倍的增加，而在对照组中是基础值的约一半。此外，在对照组和益生菌组中，向大肠添加葡萄糖后的Δ Isc大于小肠中的Δ Isc。此外，在空肠和结肠中，饲喂乳酸杆菌的鸡的Isc的增加高于其对照对应物（p < 0.1）。这一结果表明，在肉鸡日粮中添加乳酸杆菌增加了葡萄糖的转运。此外，结果表明，空肠和结肠组织的电导率保持不受乳酸杆菌的膳食包含的影响，并支持这种添加剂增强上皮屏障的维持和功能的概念。总之，日粮中添加微生物饲料添加剂（L。salivarius和L. reuteri）略微提高了生长性能并改善了与肠道结构改善相关的肠道营养吸收。
P>Probiotics might be one of the solutions to reduce the effects of the recent ban on antimicrobial growth promoters in feed. However, the mode of action of probiotics still not fully understood. Therefore, evaluating probiotics (microbial feed additives) is essential. Thus the objective of this work was to investigate the efficacy of a new microbial feed additive (Lactobacillus salivarius and Lactobacillus reuteri) in broiler nutrition. The body weight (BW), average daily weight gain was relatively increased by the dietary inclusion of Lactobacillus sp. in broiler diets. Furthermore, the Lactobacillus feed additive influenced the histomorphological measurements of small intestinal villi. The addition of Lactobacillus sp. increased (p < 0.05) the villus height (VH)/crypt depth ratio and the VH was numerically increased in duodenum. The duodenal crypt depth remained unaffected (p > 0.05), while the ileal crypt depth was decreased by dietary supplementation of Lactobacillus sp. compared with the control. At the end of the feeding period, the basal and glucose stimulated short-circuit current (Isc) and electrical tissue conductivity were measured in the isolated gut mucosa to characterize the electrical properties of the gut. The addition of glucose on the mucosal side in Ussing chamber produced a significant increase (p = 0.001) in Isc in both jejunum and colon relative to the basal values in Lactobacillus probiotic group. This increase in Isc for probiotic group in jejunum is equivalent to an increase of about two times that for the basal values, while in the control group is about half fold that for the basal value. In addition, the Delta Isc after glucose addition to the large intestine was greater than the Delta Isc in the small intestine in both control and probiotic group. Moreover in both jejunum and colon, the increase in Isc for birds fed Lactobacillus was higher than their control counterparts (p < 0.1). This result suggests that the addition of Lactobacillus sp. to broiler diets increased the glucose transport. Additionally, the results indicated that the conductivity of jejunal and colonic tissues remained unaffected by the dietary inclusion of Lactobacillus and support the concept that this additive enhances the maintenance and function of the epithelial barrier. In conclusion, dietary inclusion of a microbial feed additive (L. salivarius and L. reuteri) slightly increased the growth performance and improved intestinal nutrient absorption which was in association with the intestinal architecture improvement.