Genome-Wide Identification and Functional Analysis of Carboxylesterase and Methylesterase Gene Families in Peach (Prunus persica L. Batsch)
Genome-Wide Identification and Functional Analysis of Carboxylesterase and Methylesterase Gene Families in Peach (Prunus persica L. Batsch)
复制标题
桃（Prunus persica L. Batsch）中羧酸酯酶和甲基酯酶基因家族的全基因组鉴定和功能分析
DOI：
10.3389/fpls.2019.01511
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发表时间：
2019-11-22
影响因子：
5.6
通讯作者：
Zhang, Bo
中科院分区：
文献类型：
--
作者：
Cao, Xiangmei;Duan, Wenyi;Zhang, Bo
羧酸酯酶(CXE)和甲基酯酶(MES)是作用于羧酸酯的水解酶，参与植物的代谢过程和防御反应。植物CXE和MES基因的一些功能已被鉴定，但关于大多数成员的作用信息很少。我们在一个重要的商业物种--桃(Prunus persica L.Batsch)中对该基因家族进行了全面研究。共鉴定出33个桃CXE基因和18个MES基因，它们在染色体上分布不均。根据系统发育分析，CXE和MESS聚为两个不同的分支。比较内含子的位置和基序的差异，揭示了CXE基因和MES基因之间的进化关系。RNA-SEQ显示CXE/Mess在桃花、叶片和成熟果实中的差异表达模式，以及对茉莉酸甲酯(MeJA)和紫外线B的响应。用实时荧光定量聚合酶链式反应验证候选基因的转录水平。在大肠杆菌中的异源表达鉴定了三个CXE，它们在体外参与了挥发酯的水解。此外，还鉴定了两种能够降解MeJA和水杨酸甲酯的重组MES蛋白。我们的结果为鉴定CXE和MES功能基因提供了重要的资源，这些基因参与了挥发酯的分解代谢、对生物和非生物胁迫的响应以及MeJA和水杨酸甲酯等信号分子的激活。
Carboxylesterases (CXE) and methylesterases (MES) are hydrolytic enzymes that act on carboxylic esters and are involved in plant metabolic processes and defense responses. A few functions of plant CXE and MES genes have been identified but very little information is available about the role of most members. We made a comprehensive study of this gene family in a commercially important species, peach (Prunus persica L. Batsch). A total of 33 peach CXE genes and 18 MES genes were identified and shown to be distributed unevenly between the chromosomes. Based on phylogenetic analysis, CXEs and MESs clustered into two different branches. Comparison of the positions of intron and differences in motifs revealed the evolutionary relationships between CXE and MES genes. RNA-seq revealed differential expression patterns of CXE/MESs in peach flower, leaf, and ripening fruit and in response to methyl jasmonate (MeJA) and ultraviolet B treatment. Transcript levels of candidate genes were verified by real-time quantitative PCR. Heterologous expression in Escherichia coli identified three CXEs that were involved in the hydrolysis of volatile esters in vitro. Furthermore, two recombinant MES proteins were identified that could hydrolyze MeJA and methyl salicylate. Our results provide an important resource for the identification of functional CXE and MES genes involved in the catabolism of volatile esters, responses to biotic and abiotic stresses and activation of signaling molecules such as MeJA and methyl salicylate.