(1)H, (13)C, (15)N backbone resonance assignment for the 1-164 construct of human XRCC4.
(1)H, (13)C, (15)N backbone resonance assignment for the 1-164 construct of human XRCC4.
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DOI：
10.1007/s12104-021-10035-6
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发表时间：
2021-10
影响因子：
0.9
通讯作者：
Cliff MJ
中科院分区：
文献类型：
--
作者：
Cabello-Lobato MJ;Schmidt CK;Cliff MJ
DNA双链断裂（DSB）代表了最具细胞毒性的DNA损伤，因为如果错配或未修复，它们会导致细胞死亡或导致基因组不稳定，这反过来又会导致癌症。DSB通过两种主要途径修复，称为同源重组和非同源末端连接（NHEJ）。NHEJ负责修复人类细胞中产生的绝大多数DSB。NHEJ因子的缺陷也与小头畸形、原始侏儒症和免疫缺陷有关。对于介导NHEJ重要的关键蛋白之一是XRCC 4。XRCC 4是二聚体，其二聚体界面由延伸的卷曲螺旋介导。N-末端头部结构域形成混合的α-β球状结构。许多因子与卷曲螺旋结构域的C末端相互作用，这也与XRCC 4二聚体之间的显著自缔合相关。表达并纯化了一系列构建体长度的人XRCC 4，并且1-164变体具有最好的NMR特性，如通过一致的线宽和化学位移分散所判断的。在这项工作中，我们报告了1-164构建体溶液形式的人XRCC 4的1H，15 N和13 C骨架共振归属。通过异向多维NMR光谱获得化合物。总共，XRCC 4的161个可分配残基中的156个被分配到TROSY光谱中的共振，另外11个共振被分配到His-标签残基。使用TALOS +网络服务器从化学位移分析预测溶液二级结构与该蛋白质的已公布的X射线晶体结构非常一致。在线版本包含补充材料，可通过10.1007/s12104-021-10035-6获得。
DNA double-strand breaks (DSBs) represent the most cytotoxic DNA lesions, as—if mis- or unrepaired—they can cause cell death or lead to genome instability, which in turn can cause cancer. DSBs are repaired by two major pathways termed homologous recombination and non-homologous end-joining (NHEJ). NHEJ is responsible for repairing the vast majority of DSBs arising in human cells. Defects in NHEJ factors are also associated with microcephaly, primordial dwarfism and immune deficiencies. One of the key proteins important for mediating NHEJ is XRCC4. XRCC4 is a dimer, with the dimer interface mediated by an extended coiled-coil. The N-terminal head domain forms a mixed alpha–beta globular structure. Numerous factors interact with the C-terminus of the coiled-coil domain, which is also associated with significant self-association between XRCC4 dimers. A range of construct lengths of human XRCC4 were expressed and purified, and the 1–164 variant had the best NMR properties, as judged by consistent linewidths, and chemical shift dispersion. In this work we report the 1H, 15 N and 13C backbone resonance assignments of human XRCC4 in the solution form of the 1–164 construct. Assignments were obtained by heteronuclear multidimensional NMR spectroscopy. In total, 156 of 161 assignable residues of XRCC4 were assigned to resonances in the TROSY spectrum, with an additional 11 resonances assigned to His-Tag residues. Prediction of solution secondary structure from a chemical shift analysis using the TALOS + webserver is in good agreement with the published X-ray crystal structures of this protein. The online version contains supplementary material available at 10.1007/s12104-021-10035-6.
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影响因子：
2.9
作者：
Vranken, WF;Boucher, W;Laue, ED
通讯作者：
Laue, ED
DOI：
10.1002/prot.340230412
发表时间：
1995-12-01
期刊：
PROTEINS-STRUCTURE FUNCTION AND GENETICS
影响因子：
--
作者：
Frishman, D;Argos, P
通讯作者：
Argos, P
影响因子：
7.7
作者：
Normanno, Davide;Negrel, Aurelie;Modesti, Mauro
通讯作者：
Modesti, Mauro
影响因子：
5.3
作者：
Wu, Pei-Yu;Frit, Philippe;Junop, Murray S.
通讯作者：
Junop, Murray S.
影响因子：
2.7
作者：
Hyberts SG;Robson SA;Wagner G
通讯作者：
Wagner G