New bacterial species associated with chronic periodontitis
New bacterial species associated with chronic periodontitis
复制标题
DOI：
10.1177/154405910308200503
复制
发表时间：
2003-05-01
影响因子：
7.6
通讯作者：
Leys, EJ
中科院分区：
文献类型：
--
作者：
Kumar, PS;Griffen, AL;Leys, EJ
最近基于16S核糖体克隆和测序对人类龈下口腔菌群的研究表明，许多细菌种类是新的物种或种型。本研究的目的是在这些新发现的物种和种型中鉴定潜在的牙周病原体。开发了物种特异性核糖体16S引物进行PCR扩增，用于检测新种。与慢性有关。新发现的菌种包括脱铁杆菌门的D084、BH017、拟杆菌门的AU126、巨生菌门的BB 166、OP11门的X112、TM7门的I025，以及已命名的隐真杆菌、牙髓卟啉单胞菌、牙齿普雷伏菌和直肠隐杆菌等。在牙周健康中较为常见的种或种型包括两种未培养的种型：脱铁杆菌门的克隆W090和拟杆菌门的克隆BU063，以及定名的种：干酪托波菌和细小托波菌。
Recent investigations of the human subgingival oral flora based on ribosomal 16S cloning and sequencing have shown many of the bacterial species present to be novel species or phylotypes. The purpose of the present investigation was to identify potential periodontal pathogens among these newly identified species and phylotypes. Species-specific ribosomal 16S primers for PCR amplification were developed for detection of new species. Associations With chronic. periodontitis were observed for several new species or phylotypes, including uncultivated clones D084 and BH017 from the Deferribacteres phylum, AU126 from the Bacteroidetes phylum, Megasphaera clone BB 166, clone X112 from the OP11 phylum, and clone I025 from the TM7 phylum, and the named species Eubacterium saphenum, Porphyromonas endodontalis, Prevotella denticola, and Cryptobacterium curtum. Species or phylotypes more prevalent in periodontal health included two uncultivated phylotypes clone W090 from the Deferribacteres phylum and clone BU063 from the Bacteroidetes, and named species Atopobium rimae and Atopobium parvulum.