Localization of B4GALNT2 and its role in mouse embryo attachment
Localization of B4GALNT2 and its role in mouse embryo attachment
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DOI：
10.1016/j.fertnstert.2012.02.019
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发表时间：
2012-05-01
影响因子：
6.7
通讯作者：
Chu, Sin Tak
中科院分区：
文献类型：
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作者：
Li, Pei-Tzu;Liao, Chi-Jr;Chu, Sin Tak
目的：研究β-1，4-N-乙酰氨基半乳糖转移酶II的定位（B4 GALNT 2）和这种蛋白质和Sd（a）抗原在胚胎植入中的参与。设计：细胞和动物研究。设置：大学。动物：成年远系繁殖癌症研究所小鼠。干预：在妊娠早期将B4 GALNT 2抗体注射到小鼠子宫中;主要观察指标：免疫荧光染色和Western blotting检测蛋白表达。结果：b4 galnt 2基因在293 T细胞中表达，其蛋白定位于细胞膜上。我们证实B4 GALNT 2定位于E3.5胚泡的表面，但在子宫上皮细胞中是细胞内组分。最后，抗B4 GALNT 2和凝集素抑制试验证实B4 GALNT 2和Sd（a）抗原通过小鼠系统和人子宫内膜细胞系（石川）参与体外和体内胚胎附着。提示B4 GALNT 2和Sda抗原在胚胎着床过程中起重要作用。（Fertil Steril（R）2012;97：1206-12. (C)美国生殖医学会（American Society for Reproductive Medicine）
Objective: To investigate the location of beta-1,4-N-acetylgalactosaminyltransferase II (B4GALNT2) and the involvement of this protein and Sd(a) antigen in embryonic implantation.Design: Cell and animal study.Setting: University.Animal(s): Adult outbred Institute for Cancer Research mice.Intervention(s): B4GALNT2 antibody injected into the uteri of mice in early pregnancy; E3.5 blastocysts and pregnant uterine tissues were collected.Main Outcome Measure(s): Protein expression was detected by immunofluorescence staining and Western blotting. Embryo attachment was assayed via in vitro and in vivo embryo implantation models.Result(s): The b4galnt2 gene expression in the 293T cell line showed the protein localized in the plasma membrane. We confirmed that B4GALNT2 was localized on the surface of E3.5 blastocysts but was an intracellular component in uterine epithelia. Finally, anti-B4GALNT2 and lectins inhibition assays demonstrated the involvement of B4GALNT2 and Sd(a) antigen in embryonic attachment in vitro and in vivo via the mouse system and human endometrial cell line (Ishikawa).Conclusion(s): B4GALNT2 expressed in the blastocyst may interact with a ligand on the endometrial surface, perhaps via Sd(a) also, to permit embryo implantation. Our data suggest that B4GALNT2 and Sda antigen are essential for embryo implantation. (Fertil Steril (R) 2012;97:1206-12. (C) 2012 by American Society for Reproductive Medicine.)