Chromatographic separation of naproxen enantiomers using hydroxypropyl-β-cyclodextrin as chiral mobile phase additive
Chromatographic separation of naproxen enantiomers using hydroxypropyl-β-cyclodextrin as chiral mobile phase additive
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DOI：
10.1080/01496390600674885
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发表时间：
2006-01-01
影响因子：
2.8
通讯作者：
Yu, JG
中科院分区：
文献类型：
--
作者：
Jiao, FP;Huang, KL;Yu, JG
本文研究了以羟丙基-β-环糊精为手性移动的添加剂的色谱分离。详细研究了流动相组成的影响。移动的相的适当组成为85：15（v/v）水溶液（含0.5%TEA，pH 3.5）/含25 mM HP-β-CD的乙醇，柱温设定为25 ℃。1/ k与[HP-β-CD]的图给出了良好的线性关系，表明萘普生与HP-β-CD的化学计量比为1：1。通过ln α与1/T的关系计算了表观热力学参数，发现对映体分离为焓驱动，包合过程为放热过程。
Chromatographic separation of naproxen has been studied using hydroxypropyl-beta-cyclodextrin as chiral mobile phase additive. The effects of mobile-phase composition were researched in detail. The appropriate composition of mobile phase was 85:15 (v/v) aqueous with 0.5% TEA at pH 3.5/ ethanol containing 25 mM HP-beta-CD, and the column temperature was set of 25 degrees C. Graphs of 1/ k versus [HP-beta-CD] gave good linear relationships, indicating the stoichiometry ratio of naproxen with HP-beta-CD of 1:1. Apparent thermodynamic parameters were also calculated from the plots of ln alpha versus 1/T, it was found that the enantioseparation was enthalpy driven and the inclusion process was exothermic.