Introducing dip pen nanolithography as a tool for controlling stem cell behaviour: unlocking the potential of the next generation of smart materials in regenerative medicine
Introducing dip pen nanolithography as a tool for controlling stem cell behaviour: unlocking the potential of the next generation of smart materials in regenerative medicine
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DOI：
10.1039/c004149a
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发表时间：
2010-01-01
期刊：
影响因子：
6.1
通讯作者：
Hunt, John A.
中科院分区：
文献类型：
--
作者：
Curran, Judith M.;Stokes, Robert;Hunt, John A.
不论其来源如何，都需要对干细胞群进行可重复控制，以实现这些细胞作为医疗疗法、细胞生物学研究工具和体外分析的真正潜力。迄今为止，由于细胞和材料的可变性，将这些细胞用于临床试验甚至简单的体外实验时，成功输出的结果缺乏一致性。以纳米阵列形式成功地结合单一化学物质来控制干细胞或任何细胞行为，这在以前还没有报道过。在这里，我们报告了均匀的纳米图案化学修饰表面如何以高度可重复的方式用于启动定向细胞反应，特别是间充质干细胞（MSC）分化，而不需要外源生物因子和大量补充的细胞培养基。这些数据的成功获取将导致材料的细胞选择特性的优化，进一步增强纳米化底物在细胞生物学和再生医学中的作用。本文报道了均质分子纳米化表面的成功设计和比较及其对人间充质干细胞粘附和分化的直接影响。利用蘸笔纳米光刻技术（DPN (R)）对平面金表面进行了图像化处理，生成了纳米点阵列，其优化的固定直径为70纳米，间隔为140至1000纳米，末端官能团包括羧基、氨基、甲基和羟基。这些纳米图案表面表现出前所未有的对初始细胞相互作用的控制和随后对细胞表型的控制，并为未来提供了巨大的潜力。
Reproducible control of stem cell populations, regardless of their original source, is required for the true potential of these cells to be realised as medical therapies, cell biology research tools and in vitro assays. To date there is a lack of consistency in successful output when these cells are used in clinical trials and even simple in vitro experiments, due to cell and material variability. The successful combination of single chemistries in nanoarray format to control stem cell, or any cellular behaviour has not been previously reported. Here we report how homogenously nanopatterned chemically modified surfaces can be used to initiate a directed cellular response, particularly mesenchymal stem cell (MSC) differentiation, in a highly reproducible manner without the need for exogenous biological factors and heavily supplemented cell media. Successful acquisition of these data should lead to the optimisation of cell selective properties of materials, further enhancing the role of nanopatterned substrates in cell biology and regenerative medicine. The successful design and comparison of homogenously molecularly nanopatterned surfaces and their direct effect on human MSC adhesion and differentiation are reported in this paper. Planar gold surfaces were patterned by dip pen nanolithography (DPN (R)) to produce arrays of nanodots with optimised fixed diameter of 70 nanometres separated by defined spacings, ranging from 140 to 1000 nm with terminal functionalities of simple chemistries including carboxyl, amino, methyl and hydroxyl. These nanopatterned surfaces exhibited unprecedented control of initial cell interactions and subsequent control of cell phenotype and offer significant potential for the future.