Prevention of pulmonary metastasis from subcutaneous tumors by binary system-based sustained delivery of catalase
Prevention of pulmonary metastasis from subcutaneous tumors by binary system-based sustained delivery of catalase
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DOI：
10.1016/j.jconrel.2009.04.005
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发表时间：
2009-07-20
影响因子：
10.8
通讯作者：
Hashida, Mitsuru
中科院分区：
文献类型：
--
作者：
Hyoudou, Kenji;Nishikawa, Makiya;Hashida, Mitsuru
过氧化氢酶递送可有效抑制活性氧（ROS）介导的肿瘤转移加速。我们以前的研究已经证明，通过聚乙二醇化增加过氧化氢酶（PEG-过氧化氢酶）的血浆半衰期显著增加其抑制小鼠实验性肺转移的效力。在本研究中，使用可生物降解的明胶水凝胶制剂来进一步增加PEG-过氧化氢酶的循环时间。将In-111-PEG-过氧化氢酶/水凝胶植入皮下组织中可使血浆中的放射性维持14天以上。然后，在具有B16-111.6/Luc细胞皮下肿瘤的小鼠中评价PEG-过氧化氢酶/水凝胶对肿瘤细胞自发性肺转移的作用，B16-111.6/Luc细胞是稳定表达荧光素酶的鼠黑素瘤细胞系。测量肺中的荧光素酶活性显示PEG-过氧化氢酶/水凝胶显著（P
Catalase delivery can be effective in inhibiting reactive oxygen species (ROS)-mediated acceleration of tumor metastasis. Our previous studies have demonstrated that increasing the plasma half-life of catalase by pegylation (PEG-catalase) significantly increases its potency of inhibiting experimental pulmonary metastasis in mice. In the present study, a biodegradable gelatin hydrogel formulation was used to further increase the circulation time of PEG-catalase. Implantation of In-111-PEG-catalase/hydrogel into subcutaneous tissues maintained the radioactivity in plasma for more than 14 days. Then, the effect of the PEG-catalase/hydrogel on spontaneous pulmonary metastasis of tumor cells was evaluated in mice with subcutaneous tumor of B16-111.6/Luc cells, a murine melanoma cell line stably expressing luciferase. Measuring luciferase activity in the lung revealed that the PEG-catalase/hydrogel significantly (P