Molecular weight of two states of cytochrome c oxidase.
Molecular weight of two states of cytochrome c oxidase.
复制标题
细胞色素c氧化酶两种状态的分子量。
DOI：
10.1016/s0021-9258(18)62585-2
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发表时间：
1970
期刊：
影响因子：
--
通讯作者：
E. Stotz
中科院分区：
文献类型：
--
作者：
B. Love;S. Chan;E. Stotz
通过Yonetani方法制备的细胞色素氧化酶显示分子量为200，000（±5%）。在碱性pH（pH 9.5至11，0.01 m磷酸盐，0.1%非离子洗涤剂）下，酶解离为分子量为100，000（±5%）的单体物质。定性结果表明，二聚体转化为单体的速度是缓慢的，但强烈的pH值依赖性。在pH 9.5和10.5之间，当在4°下孵育相对较短（≤10小时）时，获得单体和二聚体的混合物，但在pH 10.5下静置24小时后，转化基本上完成。虽然从Archibald研究中可以获得似乎是单分散的单体制剂，但在中性pH下分离的二聚体含有少量且可能不同量的单体。
Cytochrome oxidase, as prepared by the Yonetani method, is shown to have a molecular weight of 200,000 (±5%). At alkaline pH (from pH 9.5 to 11, 0.01mphosphate, 0.1% nonionic detergent) the enzyme dissociates to a monomer species with a molecular weight of 100,000 (±5%). Qualitative results are presented to show that the rate of conversion of dimer to monomer is slow but strongly pH dependent. Between pH 9.5 and 10.5 a mixture of monomer and dimer is obtained when incubation at 4° is relatively short (≤10 hours), but the conversion is essentially complete upon standing at pH 10.5 for 24 hours. Although monomer preparations can be obtained which appear to be mono-disperse from Archibald studies, the dimer isolated at neutral pH contains a small and probably variable amount of the monomer.