The effect of interleukin-1β, interleukin-6, and tumor necrosis factor-α on estradiol-17β release in the myometrium: The in vitro study on the pig model
The effect of interleukin-1β, interleukin-6, and tumor necrosis factor-α on estradiol-17β release in the myometrium: The in vitro study on the pig model
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DOI：
10.1016/j.theriogenology.2013.09.024
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发表时间：
2014-01-15
期刊：
影响因子：
2.8
通讯作者：
Kotwica, G.
中科院分区：
文献类型：
--
作者：
Franczak, A.;Wojciechowicz, B.;Kotwica, G.
雌二醇-17β(E-2)是猪早期妊娠和发情周期的有效调节剂。E-2的产生存在于猪子宫肌层，但参与其调控的因素尚不清楚。在体外实验中，研究了妊娠10~11天、12~13天、15~16天和发情周期的猪子宫肌层中IL-1β、IL-6和肿瘤坏死因子(TNF)-α对E-2释放的影响。细胞色素P450家族19(CyP19)基因的表达和芳香酶细胞色素P450蛋白在子宫肌层中的存在证实了该组织产生E-2的能力。在妊娠猪中，IL1RI和IL6R的表达在妊娠15~16天显著增加，而TNFRI的表达在妊娠10~11天显著增加。在妊娠猪中，IL-1β、IL-6和TNF-α在妊娠15~16天可促进子宫肌层E-2的分泌，但对妊娠10~11天和12~13天的E-2分泌无明显影响。在周期猪中，IL-1β、IL-6和肿瘤坏死因子-α不增加子宫肌层E-2的释放。总之，IL-1β、IL-6和TNF-α以一种依赖于女性生理状态的方式影响子宫肌层E-2的释放。猪子宫肌层表达IL1RI、IL6R和TNFRI基因，是IL-1β、IL-6和TNF-α的靶组织。在妊娠猪中，IL-1β、IL-6和TNF-α在体外可能会增加子宫肌层中E-2的释放，特别是在怀孕15至16天。这些发现可能会让研究人员感兴趣，他们将猪作为胎儿编程的动物模型。(C)2014 Elsevier Inc.保留所有权利。
Estradiol-17 beta (E-2) is a potent regulator of early pregnancy and the estrous cycle in pigs. Production of E-2 occurs in the porcine myometrium, but the factors involved in its regulation are unknown. In this in vitro study, it was investigated whether interleukin (IL)-1 beta, IL-6, and tumor necrosis factor (TNF)-alpha affect the release of E-2 from the porcine myometrium on Days 10 to 11, 12 to 13, and 15 to 16 of pregnancy and the estrous cycle. The expression of the cytochrome P450 family 19 (CYP19) gene and the presence of the aromatase cytochrome P450 protein in the myometrium confirmed the ability of the tissue to produce E-2. In gravid pigs, the expression of IL1RI mRNA and IL6R mRNA was markedly increased on Days 15 to 16 of gestation, whereas TNFRI mRNA was increased on Days 10 to 11 of gestation. In cyclic pigs, the expression of myometrial IL1RI mRNA did not differ among the studied days, although the expression of IL6R and TNFRI mRNAs was increased on Days 15 to 16. In gravid pigs, IL-1 beta, IL-6, and TNF-alpha increased myometrial E-2 secretion on Days 15 to 16 but did not affect E-2 release on Days 10 to 11 and 12 to 13 of pregnancy. In cyclic pigs, IL-1 beta, IL-6, and TNF-alpha did not increase myometrial E-2 release. In conclusion, IL-1 beta, IL-6, and TNF-alpha affected myometrial E-2 release in a manner that is dependent on the physiologic status of the female. The porcine myometrium expresses IL1RI, IL6R, and TNFRI genes and is the target tissue for IL-1 beta, IL-6, and TNF-alpha. In gravid pigs, IL-1 beta, IL-6, and TNF-alpha may increase myometrial release of E-2 in vitro specifically on Days 15 to 16 of pregnancy. These findings may be of interest to researchers using pigs as an animal model for fetal programming. (C) 2014 Elsevier Inc. All rights reserved.