Medicago truncatula, a model plant for studying the molecular genetics of the Rhizobium-legume symbiosis.
Medicago truncatula, a model plant for studying the molecular genetics of the Rhizobium-legume symbiosis.
复制标题
DOI：
10.1007/bf02668879
复制
发表时间：
1990-01-01
影响因子：
2.1
通讯作者：
Huguet, T.
中科院分区：
文献类型：
--
作者：
Barker, D. G.;Bianchi, S.;Huguet, T.
M. truncatula是二倍体和自花受精，具有相对较小的基因组，并且初步分子分析表明与异交四倍体M相比等位基因杂合性最小。sativa. M.蒺藜Jemalong是由R.苜蓿属菌株2011，其已经用于定义参与与卢塞恩共生的许多细菌基因。鉴定了Jemalong的基因型，其可以在农杆菌转化后再生，从而允许在同源转基因系统中分析体外修饰的基因。
M. truncatula is diploid and autogamous, has a relatively small genome and preliminary molecular analysis suggested that allelic heterozygosity is minimal compared with the cross-fertilizing tetraploid M. sativa. M. truncatula cv. Jemalong is nodulated by the R. meliloti strain 2011, which has already served to define many of the bacterial genes involved in symbiosis with lucerne. A genotype of Jemalong was identified which can be regenerated after transformation by Agrobacterium, thus allowing the analysis of in vitro-modified genes in an homologous transgenic system.