Cdc42 couples septin recruitment to the axial landmark assembly via Axl2 in budding yeast.
Cdc42 couples septin recruitment to the axial landmark assembly via Axl2 in budding yeast.
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Cdc42 在芽殖酵母中通过 Axl2 将 septin 募集与轴向标志物组装结合起来。
DOI：
10.1101/2023.08.25.554823
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Park,Hay-Oak
中科院分区：
文献类型：
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作者：
Kang,PilJung;Mullner,Rachel;Lian,Kendra;Park,Hay-Oak
细胞极化通常发生在由空间线索引导的单一轴上。发芽酵母的细胞通过选择特定的芽位在空间上进行极化生长和定向细胞分裂。单倍体α细胞在包括Bud3、Bud4、Axl1和Axl2的瞬时标志的响应下，以轴向模式萌发。Septins是一类形成丝状GTP结合蛋白的家族，也参与轴向萌发，并被招募到萌芽的初始位置，但招募的机制尚不清楚。在这里，我们证明了Axl2以GTP结合的状态与Bud3和Cdc42 GTP酶相互作用。Axl2还与cdc10相互作用，cdc10是一种间隔素亚单位，促进细胞分裂部位附近的间隔素有效率地募集。此外，在半允许的温度下，轴向萌发模式缺陷的acdc42突变体与Axl2的相互作用减少，并损害了在G1期的Septin募集。因此，我们认为活性的CDC42将Axl2带到Bud3-Bud4复合体，然后Axl2与Cdc10相互作用，将Septin的募集与轴向里程碑联系起来。
Cell polarization generally occurs along a single axis that is directed by a spatial cue. Cells of the budding yeastSaccharomyces cerevisiaeundergo polarized growth and oriented cell division in a spatial pattern by selecting a specific bud site. Haploidaor α cells bud in the axial pattern in response to a transient landmark that includes Bud3, Bud4, Axl1 and Axl2. Septins, a family of filament-forming GTP-binding proteins, are also involved in axial budding and are recruited to an incipient bud site, but the mechanism of recruitment remains unclear. Here, we show that Axl2 interacts with Bud3 and the Cdc42 GTPase in its GTP-bound state. Axl2 also interacts with Cdc10, a septin subunit, promoting efficient recruitment of septins near the cell division site. Furthermore, acdc42mutant defective in the axial budding pattern at a semi-permissive temperature had a reduced interaction with Axl2 and compromised septin recruitment in the G1 phase. We thus propose that active Cdc42 brings Axl2 to the Bud3–Bud4 complex and that Axl2 then interacts with Cdc10, linking septin recruitment to the axial landmark.